Protein Info for GFF1930 in Variovorax sp. SCN45

Annotation: RTX toxin transporter, ATP-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 747 transmembrane" amino acids 178 to 199 (22 residues), see Phobius details amino acids 210 to 231 (22 residues), see Phobius details amino acids 288 to 312 (25 residues), see Phobius details amino acids 318 to 338 (21 residues), see Phobius details PF03412: Peptidase_C39" amino acids 29 to 152 (124 residues), 47.9 bits, see alignment E=1.8e-16 TIGR01846: type I secretion system ATPase" amino acids 29 to 730 (702 residues), 1006.5 bits, see alignment E=3.1e-307 PF00664: ABC_membrane" amino acids 180 to 446 (267 residues), 199 bits, see alignment E=1.9e-62 PF00005: ABC_tran" amino acids 509 to 658 (150 residues), 125.9 bits, see alignment E=2.7e-40

Best Hits

Swiss-Prot: 60% identical to RTX1B_ACTPL: Toxin RTX-I translocation ATP-binding protein (apxIB) from Actinobacillus pleuropneumoniae

KEGG orthology group: K11004, ATP-binding cassette, subfamily B, bacterial HlyB/CyaB (inferred from 81% identity to aaa:Acav_2038)

Predicted SEED Role

"cyclolysin secretion ATP-binding protein" in subsystem cAMP signaling in bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (747 amino acids)

>GFF1930 RTX toxin transporter, ATP-binding protein (Variovorax sp. SCN45)
MGIGEGVVQQHGVERARPQEAPDAGAPSLIALCTIARFHQIAADASLLSHQLGLASSEPI
NTATLLGAAKHLGLKAKSSRTTLDRLSLTPLPALAVLRDESGQERFVILAQCDAQRVLLQ
DPSSSNGRPVIEPLEVFAAHWTGELILITSRASLAGDLAKFDFSWFIPSLVKHRKLLGEV
LFISFILQLFALVSPLFFQVVMDKVLVHRGLTTLDVLVIGLLVVVVFESLLNGLRSYVFS
HTTNRIDVELGARLFRHLVQLPLAYFQARRVGDSVARVRELENIRSFLTGNALTVLLDVV
FSIVFVAVMLFYSVPLTLIVLASMPLYFGLSLAVVPILRSRLDEKFARGAENQAMLVETV
SAIQTVKATALEPAFGRRWDNQLAAYISASFRTQNLASWANEGVNLIGKLVNAATLWYGA
HLVMNNDLTVGQFVAFNMFAQRVSQPIMRMAQLWTDFQQTGISMARLGDILNTRTEVPPS
TAAQLPALKGRVTLDNLTFRYRPEAAPVLNGVSLDVRPGEVIGIVGRSGSGKSTLTKLIQ
RLYAPEQGRLLVDGIDISLIDAAQLRMQVGVVLQENTLFNRSVRENIAIVDPAAPLEAII
HAAQLAGAHEFISELPEGYDTMVGEQGASLSGGQRQRIAIARALFTHPRVLIFDEATSAL
DYESEAIVQRNMAHICRGRTVFIIAHRLSAVRHANRIIVMDKGKIVEGGTHESLLARPKG
IYAHLWNMQGGGHPTETSDGAGSGAPA