Protein Info for GFF1913 in Sphingobium sp. HT1-2

Annotation: ATP-dependent helicase HrpB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 816 TIGR01970: ATP-dependent helicase HrpB" amino acids 6 to 809 (804 residues), 910.7 bits, see alignment E=4.9e-278 PF00270: DEAD" amino acids 17 to 162 (146 residues), 45.3 bits, see alignment E=1.7e-15 PF00271: Helicase_C" amino acids 204 to 326 (123 residues), 50.9 bits, see alignment E=3.5e-17 PF04408: WHD_HA2" amino acids 389 to 417 (29 residues), 22.6 bits, see alignment (E = 1.8e-08) PF08482: HrpB_C" amino acids 666 to 798 (133 residues), 161.5 bits, see alignment E=2.8e-51

Best Hits

KEGG orthology group: K03579, ATP-dependent helicase HrpB [EC: 3.6.4.13] (inferred from 86% identity to sjp:SJA_C1-27700)

Predicted SEED Role

"ATP-dependent helicase HrpB"

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (816 amino acids)

>GFF1913 ATP-dependent helicase HrpB (Sphingobium sp. HT1-2)
MTAPALPIHAVLPDLLAALARGSNAVLVAPPGAGKTTAVAPALLDQPWCEGQVLLLSPRR
LAARAAAERIAEMLGEQPGGTVGYATRMDSKVSAKTRLLVLTEGIFVRRIQDDPELAGVS
AVLFDEVHERSLDSDFGLALALDAQAALRPDLRILPMSATLDGARFSTLLDGAPVIESEG
KIQPLELRHIGRHAEKRIEDEMAAAIRRALEEETQGDLLAFLPGVREIERTAERIEGGAF
EVHMLHGSLDPAAQRAAIRPSREGRRKVILATSIAETSLTIDGVRIVVDSGLARRPRYDR
AAGVTRLVTERASQASATQRAGRAARQRPGVAYRLWEAAATAGMPPFDPPEILESDLSSL
LLDCALWGVSDPASLRWLDAPPAAAVAEAAKRLTALEALDADGRITAHGKALATLPLAPR
IAHMLVRAGERGLARTAAEIAVLLGERGLGGQDTDLTLRFQRWRREGGKRAEAGRGLAKR
WARLVDSRSSAEGGLHDVGLCLALAFPDRVAKRRSADGADWASVGGRGFRLDPLSPLASE
TWLAVGEVQGSAAGARILSAAPIGEAEVIALFGERIAEHRTVKFRPANGGIEALRERRLG
AVRLSSGSDDRPDPDAVAAALLDGVRQGGLALLPWSEAAQSLRMRADFAGIEALSDAALI
DTLDEWLPPLLAGKRRLSDIDRSQLSGVLETLIGWDGKQQLDRLAPPDFRSPAGSTHAID
YAAEGGPRVELRVQALFGLSQHPTVGNQRIPLVLSLTSPAGRPIQTTRDLPGFWAGNWRD
VAKEMRGRYPRHPWPDDPASANATLRTKNADARRNS