Protein Info for GFF191 in Sphingobium sp. HT1-2

Annotation: Conjugative transfer protein TrbE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 816 transmembrane" amino acids 672 to 693 (22 residues), see Phobius details PF03135: CagE_TrbE_VirB" amino acids 306 to 389 (84 residues), 29.9 bits, see alignment E=5.4e-11 PF19044: P-loop_TraG" amino acids 578 to 730 (153 residues), 26.1 bits, see alignment E=3.4e-10

Best Hits

KEGG orthology group: K03199, type IV secretion system protein VirB4 (inferred from 73% identity to sjp:SJA_C1-29670)

Predicted SEED Role

"Conjugative transfer protein TrbE" in subsystem Type 4 secretion and conjugative transfer

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (816 amino acids)

>GFF191 Conjugative transfer protein TrbE (Sphingobium sp. HT1-2)
MLSLREYRNRSERLADHLPWAALIAPGIVLNKDGSFLRILAFRGPDLESATEAELVSACA
RANNALKRLGSGWALFFDAERRESHAYPQCAFPDAASWLVEAERRAAFQAEGTHFESRYH
LGLTWLPPGDAQDSAGRSLVERPDADSGRDWRGALAQFITETDRVLDLLSGFMADVRPLD
DDATLTFLHGTVSDRRHPVSTPATPMYLDGVLADTPLVGGLEPRLGDLHVRTLTILGFPS
LSRPGILDALNHQDFGYRWVTRFIALDKTLSTKTLTSLRRQWFNKRKSITALLREVLYNS
PAQLLDSDADNKVVDADEALQALGGDHVAFGYLTATITVTDTQRGAVEEKVRRVERIVNG
LGFTCVREGANAVEAWLSSLPGQVYANVRQPLVHTLNLAHLMPLSAVWAGPEENRHLAGP
PLLIAKTAGSTPFRLSTHVGDVGHMMVVGPTGAGKSVLLSLIALQFRRYPDAQVYIFDKG
NSARAAVLAMGGAHHALGSMEADGGALAFQPLAGIDDMAERGWAAEWIAALLEHERVLVD
PAVKDAVWTALGSLASAPREERTMTGLALLLQSNALRIALQPYTLEGPHGPLLDAAEQHL
SFADVQCFETEALMEMGAAVAPVLTYLFHRLEERFDGRPTLLILDEAWIFLDHPLFAARI
REWLKVLRKKNVAVLFATQSLADIAGSAIAPAIIESCPQRILLPNDRAIEPQGREIYARF
GLNDRQVELIARATPKRHYYLQSARGNRLFELGLGPVALALCGASDPMAQARIDAVLAEH
GHGPFAQAFLRACELEWAADLVAGFPHFQPPREGEE