Protein Info for GFF1904 in Variovorax sp. SCN45

Annotation: Uncharacterized MFS-type transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 430 transmembrane" amino acids 41 to 65 (25 residues), see Phobius details amino acids 78 to 99 (22 residues), see Phobius details amino acids 111 to 131 (21 residues), see Phobius details amino acids 138 to 157 (20 residues), see Phobius details amino acids 167 to 188 (22 residues), see Phobius details amino acids 196 to 218 (23 residues), see Phobius details amino acids 248 to 273 (26 residues), see Phobius details amino acids 282 to 302 (21 residues), see Phobius details amino acids 313 to 334 (22 residues), see Phobius details amino acids 340 to 362 (23 residues), see Phobius details amino acids 374 to 396 (23 residues), see Phobius details amino acids 403 to 422 (20 residues), see Phobius details PF00083: Sugar_tr" amino acids 44 to 184 (141 residues), 26.5 bits, see alignment E=5.9e-10 amino acids 275 to 389 (115 residues), 33.3 bits, see alignment E=5.2e-12 PF07690: MFS_1" amino acids 45 to 314 (270 residues), 129.8 bits, see alignment E=2.4e-41 amino acids 284 to 424 (141 residues), 71.3 bits, see alignment E=1.5e-23 PF12832: MFS_1_like" amino acids 55 to 406 (352 residues), 64.8 bits, see alignment E=1.6e-21 PF05977: MFS_3" amino acids 93 to 417 (325 residues), 27.7 bits, see alignment E=2e-10

Best Hits

Swiss-Prot: 41% identical to MDTG_ERWBE: Multidrug resistance protein MdtG (mdtG) from Erwinia billingiae (strain Eb661)

KEGG orthology group: None (inferred from 91% identity to vpe:Varpa_4489)

Predicted SEED Role

"Multidrug-efflux transporter, major facilitator superfamily (MFS) (TC 2.A.1)" in subsystem Multidrug Resistance Efflux Pumps

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (430 amino acids)

>GFF1904 Uncharacterized MFS-type transporter (Variovorax sp. SCN45)
MGKRAIGRAATQWIRPIMTNPTTPDAPAAHTGTDTHWQRNLAVCMFGSFTTIIAMTLLLP
FLPLYVEQLGVSDHAAIVQWSGAAYGATFFSAALVAPLWGRLADRYGRKPMLIRASLGMS
VAMALIGIAGNVWQLVGLRLLAGLLGGYASGSMVLVATQTPKARTGWALGTMSSAIMAGN
LVGPLVGGVLPPLIGIRATFFAAGAMIFVAFLATAFLIREEKKTPGASKQRGTADAGWAA
VPDKRPLVAMLLTGMLLMLANMSIEPIITVYVATLVEDPNRVTMMAGLVMSAAALGSILS
ASRLGKLADRVGHWNVIVGCLAVSAVLLVPQAFVTSGWQLVVLRFLMGLSLGGLLPCIAS
VIRHNVPERVAGNMLGYSTSAQYTGQVAGPLLGGFVGGHVGMRAVFLGTCVLMAAGAGWN
WLAKRRQPPG