Protein Info for GFF1889 in Variovorax sp. SCN45

Annotation: Aerobic C4-dicarboxylate transporter for fumarate, L-malate, D-malate, succunate

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 462 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details amino acids 36 to 36 (1 residues), see Phobius details transmembrane" amino acids 21 to 35 (15 residues), see Phobius details amino acids 48 to 72 (25 residues), see Phobius details amino acids 84 to 105 (22 residues), see Phobius details amino acids 154 to 172 (19 residues), see Phobius details amino acids 191 to 213 (23 residues), see Phobius details amino acids 220 to 249 (30 residues), see Phobius details amino acids 265 to 283 (19 residues), see Phobius details amino acids 303 to 327 (25 residues), see Phobius details amino acids 337 to 362 (26 residues), see Phobius details amino acids 373 to 397 (25 residues), see Phobius details PF00375: SDF" amino acids 17 to 406 (390 residues), 329.7 bits, see alignment E=1.3e-102

Best Hits

Swiss-Prot: 48% identical to DCTA3_BRADU: C4-dicarboxylate transport protein 3 (dctA3) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: None (inferred from 84% identity to rsl:RPSI07_mp0966)

Predicted SEED Role

"Aerobic C4-dicarboxylate transporter for fumarate, L-malate, D-malate, succunate" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (462 amino acids)

>GFF1889 Aerobic C4-dicarboxylate transporter for fumarate, L-malate, D-malate, succunate (Variovorax sp. SCN45)
MTTMQAPKPLPWYRKLGMQVLLSLVLGIAVGFVFPKFASQLKLLGDMFLSLIKAGVAPLV
FLTIVHGIASAGDVKSAGRVGWRAILYFEVVSTIALVVGLFAGNLLGIGKGMATVAGGAA
APAAAKAAPPQGFLEFMAHIVPDNFVGAFAKGELLQVVVLAVMVGIGILAIPDARRARIN
EGLDNISEVLFSFINLVMKLAPIGTFGAVAYSVGSNGTAVLVALAQLVLSFYAVVFLFIA
VVMGLIARLAGFSLWRFLRFIKDEILIVLGTASSESALPRLLIKLERLGCAKQTVGLVLP
TGYAFNLDGTSLFMAMGVMFISHAYGVPLTLDHQIGILLLMLLTSKGAATVSGGSFVVFA
ATVTSTGMLPVEGLAVIFGVYRFLSMAIATCNTIGNSVATVVVAKWSGTFDAKTAERHLY
PERHPDAAAADAALADGNLLNEDARDANPHPGGIPTSRPHPV