Protein Info for GFF1887 in Variovorax sp. SCN45

Annotation: Fumarate hydratase class II (EC 4.2.1.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00206: Lyase_1
  • amino acids 13 to 342 (330 residues), 297.2 bits, E=1.6e-92
• PF10415: FumaraseC_C
  • amino acids 409 to 454 (46 residues), 55 bits, 9.2e-19

Best Hits

Swiss-Prot: 53% identical to FUMC2_BRADU: Fumarate hydratase class II 2 (fumC2) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: None (inferred from 74% identity to vei:Veis_4596)

MetaCyc: 53% identical to fumarase (Arabidopsis thaliana col)
Fumarate hydratase. [EC: 4.2.1.2]

Predicted SEED Role

"Fumarate hydratase class II (EC 4.2.1.2)" in subsystem TCA Cycle (EC 4.2.1.2)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (24/26 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (20/22 steps found)
• TCA cycle II (plants and fungi) (9/9 steps found)
• TCA cycle V (2-oxoglutarate synthase) (9/9 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• TCA cycle III (animals) (9/10 steps found)
• TCA cycle VIII (Chlamydia) (6/6 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (8/9 steps found)
• partial TCA cycle (obligate autotrophs) (7/8 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (13/17 steps found)
• TCA cycle VI (Helicobacter) (7/9 steps found)
• TCA cycle VII (acetate-producers) (7/9 steps found)
• mixed acid fermentation (11/16 steps found)
• reductive TCA cycle I (7/11 steps found)
• incomplete reductive TCA cycle (4/7 steps found)
• pyruvate fermentation to propanoate I (4/7 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (6/10 steps found)
• reductive TCA cycle II (7/12 steps found)
• methylaspartate cycle (12/19 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (7/13 steps found)
• L-glutamate degradation VIII (to propanoate) (4/11 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (22/56 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.2

Use Curated BLAST to search for 4.2.1.2

Protein Sequence (460 amino acids)

>GFF1887 Fumarate hydratase class II (EC 4.2.1.2) (Variovorax sp. SCN45)
MTAMRIEHDAFGPVEIPAGKYWGAQTQRAASVFEVGTERFPACLLHAFGLQKIAAARANE
RCGALDAALAAQIVVAAEELSRGLFDAHFPLTVWQTGSGTQTNMNANEVIANRANELLGQ
PLGTKSPVHPNDHVNASQSSNDSFPTVMHIAAAMELTGRLLPSLAVLKSALEERALAFAG
AVKVARTHLMDAVPMTMGQAFDAFAWQVGNGMARLQDALPRLLVVAQGGTAAGTGLNAPK
NFDVLFCEELVGLTNLQFKPHPCKFEGMSGHDALVELSGALNVVATSLMKIANDIRWMGS
GPRCGIGELVVPDDGLTSSIMPGKRNPTIAEVLAQAAMQVIGNHVTVTMAGASGNFELNV
AKPVLIHNVLQSIRVMADSARVFAGKLVAGLEVDRERLASNLGNALLAVTALNPVLGYDK
VARITRTAMERGISPRDAAVELGLLSAETYDRLMAESLPR