Protein Info for GFF1879 in Variovorax sp. SCN45

Annotation: dicarboxylic acid transporter PcaT

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 448 transmembrane" amino acids 35 to 63 (29 residues), see Phobius details amino acids 75 to 97 (23 residues), see Phobius details amino acids 109 to 130 (22 residues), see Phobius details amino acids 136 to 156 (21 residues), see Phobius details amino acids 177 to 196 (20 residues), see Phobius details amino acids 208 to 227 (20 residues), see Phobius details amino acids 258 to 277 (20 residues), see Phobius details amino acids 296 to 314 (19 residues), see Phobius details amino acids 326 to 345 (20 residues), see Phobius details amino acids 351 to 374 (24 residues), see Phobius details amino acids 385 to 407 (23 residues), see Phobius details amino acids 419 to 438 (20 residues), see Phobius details PF00083: Sugar_tr" amino acids 37 to 234 (198 residues), 97.2 bits, see alignment E=1.6e-31 amino acids 240 to 434 (195 residues), 47.4 bits, see alignment E=2.1e-16 TIGR00883: MFS transporter, metabolite:H+ symporter (MHS) family protein" amino acids 43 to 432 (390 residues), 463.4 bits, see alignment E=3.5e-143 PF07690: MFS_1" amino acids 70 to 396 (327 residues), 97.3 bits, see alignment E=1.4e-31 amino acids 284 to 440 (157 residues), 44.3 bits, see alignment E=1.8e-15

Best Hits

Swiss-Prot: 69% identical to KGTP_ECOLI: Alpha-ketoglutarate permease (kgtP) from Escherichia coli (strain K12)

KEGG orthology group: K03761, MFS transporter, MHS family, alpha-ketoglutarate permease (inferred from 93% identity to vap:Vapar_3945)

MetaCyc: 69% identical to alpha-ketoglutarate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-23

Predicted SEED Role

"dicarboxylic acid transporter PcaT" in subsystem Protocatechuate branch of beta-ketoadipate pathway

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (448 amino acids)

>GFF1879 dicarboxylic acid transporter PcaT (Variovorax sp. SCN45)
MTAFASSATAGASSPDATADQNTPYTFEEKRKRIFAIFAASSGNLVEWFDFYVYAFCAIY
FAPAFFPKSDPTVQLLNTAGVFAAGFLMRPIGGWLFGRLADRKGRKTSMVTSVVMMCVGS
LVIACLPTYAQIGAAAPALLLLARLLQGLSVGGEYGTTATYMSEVAMRGQRGFFSSFQYV
TLIGGQLLAVVVVVVLQQVLDEAELRSWGWRIPFVLGAVAAIVALMLRRTLTETASTKTR
AAKGAGSVAELFKHHKRAFFVVLGYTAGGSLIFYTFTTYMQKYLVNSAGMSIKTASNIMT
ACLFIYMCMQPLFGALSDRIGRRSNMLLFGALGVLMTVPVLTSLQNVSSPLMAGVLITLA
LAVVSFYTSISGIVKAEMFPPEVRALGVGLSYAVGNAIFGGSAEYVALGLKSIGHESYFY
WYVTGMMALAFLVSLLLPKQATYLHNDK