Protein Info for GFF1875 in Xanthobacter sp. DMC5

Annotation: Lysine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 558 PF01921: tRNA-synt_1f" amino acids 29 to 382 (354 residues), 417.6 bits, see alignment E=2e-129 TIGR00467: lysine--tRNA ligase" amino acids 40 to 549 (510 residues), 163.2 bits, see alignment E=4.3e-52

Best Hits

Swiss-Prot: 74% identical to SYK_BRUSU: Lysine--tRNA ligase (lysS) from Brucella suis biovar 1 (strain 1330)

KEGG orthology group: K04566, lysyl-tRNA synthetase, class I [EC: 6.1.1.6] (inferred from 88% identity to azc:AZC_0380)

Predicted SEED Role

"Lysyl-tRNA synthetase (class I) (EC 6.1.1.6)" (EC 6.1.1.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.6

Use Curated BLAST to search for 6.1.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (558 amino acids)

>GFF1875 Lysine--tRNA ligase (Xanthobacter sp. DMC5)
MSSSEASLAPADPAQLAAELKTVAETATAWPFEEARKLVERLKKRPKEEVLFETGYGPSG
LPHIGTFGEVARTTMVRHAFRILTEDKVKTRLLCFSDDMDGMRKIPENVPDPAALVPYLQ
MPLTKVPNPFGGDYPSFGAHNNAMLRRFLDTFGFDYEFASATEYYFSGKLDAVLLKAAEK
YDEIMAVMLPTLGEERQATYSPFLPISPTSGRVLYVPLKKVDAKAGTITFDDEDGTELTL
PVTGGKVKLQWKPDFGARWAALDVDFEMFGKDHQTNAPIYDKICHILGGTAPEHFVYELF
LDEKGQKISKSKGNGLTIDEWLTYASPESLALYMFQSPRSAKKLHFDVIPKAVDEYFNFL
ARYPQQDWKLRFGNPVYHIHAGNPPDVDMPVSFTMLLNLASASNTEDESVLWGFLKRHVP
GVSDQTHPKLHELVGYAVRYFHDFVKPNKVFRTPDEVERDALTKLDAALAALPAGASADD
IQTVLYDVARPIPRYQDLKAKGATPERPGVSVEWFNTLYQVLLGLGRGPRFGSFVAIYGV
AETRELIRKALAGELAAA