Protein Info for GFF1870 in Variovorax sp. SCN45

Annotation: Coenzyme A transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01144: CoA_trans
  • amino acids 8 to 240 (233 residues), 84.3 bits, E=4.3e-28

Best Hits

Swiss-Prot: 41% identical to CARA_ACEWO: Caffeate CoA-transferase (carA) from Acetobacterium woodii

KEGG orthology group: None (inferred from 72% identity to bja:bll3298)

MetaCyc: 45% identical to Pct (Anaerotignum propionicum)
Propionate CoA-transferase. [EC: 2.8.3.1]; 2.8.3.1 [EC: 2.8.3.1]

Predicted SEED Role

"Acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit (EC 2.8.3.8)" in subsystem Acetyl-CoA fermentation to Butyrate (EC 2.8.3.8)

MetaCyc Pathways

• acetyl-CoA fermentation to butanoate (6/7 steps found)
• acetoacetate degradation (to acetyl CoA) (2/2 steps found)
• 4-aminobutanoate degradation V (5/7 steps found)
• propanoate fermentation to 2-methylbutanoate (4/6 steps found)
• pyruvate fermentation to acetone (3/5 steps found)
• (R)-lactate fermentation to propanoate II (acrylate pathway) (2/4 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (6/10 steps found)
• L-alanine degradation VI (reductive Stickland reaction) (3/6 steps found)
• superpathway of L-alanine fermentation (Stickland reaction) (5/9 steps found)
• L-glutamate degradation XI (reductive Stickland reaction) (3/7 steps found)
• succinate fermentation to butanoate (3/7 steps found)
• 1,2-propanediol biosynthesis from lactate (engineered) (2/6 steps found)
• gallate degradation III (anaerobic) (5/11 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (6/13 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (8/17 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Butanoate metabolism
• Propanoate metabolism
• Pyruvate metabolism
• Styrene degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.3.1 or 2.8.3.8

Protein Sequence (533 amino acids)

>GFF1870 Coenzyme A transferase (Variovorax sp. SCN45)
MIDCITPDAAAALLRDGDMVLAGGNGGSGVPEAVFEAIERRFTAGNGPHDLTLFHVTGVG
ALTEKGMCHFAHPGLVRRVIGGNFGMQLPFMKMILAQDVEAYNFPQGVMTHLCRAMAGRQ
PGVLTHVGLETYMDPRQDGGRMNARTTEELVELVHVAGRDWLFYRAPGVPKVALIRGTSA
DEDGYVSMEHEATAREDLSMAQAVHNAGGIVICQVKRIVKRGTLHPHMVKIPGFLIDHFV
VEPEQLQTYTTRYDPARCGEIVVPASSIAPDPLDVRRVVARRAAFELRPRDVVNLGVGIS
AMIPNVASEEGIADLITLTVESGVVGGVPGHAREFGTSVNPRVILDQSYQFDFYDGGGLS
CAFLSFAQVDPQGNVNVTRFGKRYDGAGGFINITQNTRRLVFNGSLTGGDFDIGVTEGKL
DIRRDGAFRKFVPAVDQISFSGRLARERGQQVSFVTDRAVFELEADGLVLTEIAPGVRLQ
EDVLDKIGFVVRIGDQLRTMDARIFRSGPMGVHDEFIAKAPRHAGGATKGEKA