Protein Info for PS417_09410 in Pseudomonas simiae WCS417
Annotation: isopropylmalate isomerase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 70% identical to LEUC1_MANSM: 3-isopropylmalate dehydratase large subunit 1 (leuC1) from Mannheimia succiniciproducens (strain MBEL55E)
KEGG orthology group: K01703, 3-isopropylmalate/(R)-2-methylmalate dehydratase large subunit [EC: 4.2.1.33 4.2.1.35] (inferred from 95% identity to pfs:PFLU2049)MetaCyc: 56% identical to isopropylmalate isomerase large subunit (Leptospira interrogans serovar Lai str. 56601)
(R)-2-methylmalate dehydratase. [EC: 4.2.1.35]; 3-isopropylmalate dehydratase. [EC: 4.2.1.35, 4.2.1.33]; 4.2.1.33 [EC: 4.2.1.35, 4.2.1.33]; RXN-7744 [EC: 4.2.1.35, 4.2.1.33]
Predicted SEED Role
"3-isopropylmalate dehydratase large subunit (EC 4.2.1.33)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 4.2.1.33)
MetaCyc Pathways
- superpathway of branched chain amino acid biosynthesis (17/17 steps found)
- L-leucine biosynthesis (6/6 steps found)
- 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
- L-isoleucine biosynthesis II (5/8 steps found)
- 2,5-xylenol and 3,5-xylenol degradation (1/13 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- C5-Branched dibasic acid metabolism
- Valine, leucine and isoleucine biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 4.2.1.33, 4.2.1.35
Use Curated BLAST to search for 4.2.1.33 or 4.2.1.35
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A1N7UD34 at UniProt or InterPro
Protein Sequence (472 amino acids)
>PS417_09410 isopropylmalate isomerase (Pseudomonas simiae WCS417) MTARTLYDKHIDSHTVCPLDDQGHVLLYIDRQVINEYTSPQAFSGLREAGRNVWRPGTAL AVVDHVNPTTPKRIAAMPDAGGARQVSYLAENCRDFGIELLDILDKRQGIEHVIAPEQGF ILPGMVIAAGDSHTTTYGALGAFGFGIGTSEIEHLLASQTLVYKRLKTMCVSVDGDLAPG LTSKDVIMALIGKIGASGATGYAIEFRGSTLDALSVEARMTICNMAVEAGARGAFMAPDE KVFAYLKGKPRAPQGELWDQALAGWRLLHSDVGAVFDQEVQLDATTLEPMVTWGTSPDQA APIGARVPDPQDVSDLILRQDMRRALNYMGLEAGMRLSDIVISHAFIGSCTNARIEDLRD AASVVRGKHVAEHVRAMIVPGSTEVRDQAEAEGLAAIFIDAGFEWRQSGCSMCLAMNDDV LAPGDRCASSTNRNFEGRQGAGARTHLMSPAMVAAAAITGRLTDIRHVGARP