Protein Info for GFF1847 in Sphingobium sp. HT1-2

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF01804: Penicil_amidase" amino acids 34 to 691 (658 residues), 427.2 bits, see alignment E=9.5e-132

Best Hits

Swiss-Prot: 53% identical to G7AC_PSEU7: Glutaryl-7-aminocephalosporanic-acid acylase from Pseudomonas sp. (strain SY-77)

KEGG orthology group: K01434, penicillin amidase [EC: 3.5.1.11] (inferred from 54% identity to hne:HNE_0221)

Predicted SEED Role

No annotation

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.1.11

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (700 amino acids)

>GFF1847 hypothetical protein (Sphingobium sp. HT1-2)
MAMRHMTIWLAALAASSAMTASVAAPSTRWGGGEILWDSFGVPHIYAKTEEGGFYGFGYA
QAQSHGNLLLRMYGESRARAAEYWGEKYESQDKWLVANDVPARSAQWFRQQTPQMQKNLE
AFAAGVNAYAAAHPDRIAPEVKVVLPLRGVDIIAHAQRLMNFGYIASDRKVLTDPSINEA
GGSNAWAVAPSRSASGKAMLLANPHLPWAPSQLTYYEAQITAPGLAIYGATQVGLPVLRF
GFNNDLGFTNTVNTMQGFTSYKLVPEGDGYRFDGKTLPFDTVTKSYKVKQADGTLKSVSF
EQKSTVHGPVFTLPDGKTTVALKVAGLDRPGVLEQYLEMGKARDWGAFEKALRKMQVVMF
NIIYADRAGHILYLDNGLLPKHASGDLKYWSAPVPGDTSATLWKDVHSYDDMPKLLDPAT
GFVQNANDPPWLATWPRALDPKSFPAYVAPVGPMSQRAQMSVKLMSQTPKISFDDFVSRK
LTTTSLMAERMLPDLLAAAAGSNDAEVQAATALLKGWDHRFEPDSRAALLFETWAGLFAP
KNFTDQSNYAVKWTLDDPLETPRGLKDPTAAVAMLKEAVAKTKQLYGAIDRPFGDVSRFH
IGDVSVPANGGFGNTGVFRTITWGPMKNGERTPVHGETWVSMVEFGTPMKAVGLMSYGNS
SQPGSWHNSDQLQLLADKKFRTLWISRADVEGHLEEKTAF