Protein Info for GFF1843 in Xanthobacter sp. DMC5

Annotation: 3-isopropylmalate dehydratase small subunit 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00171: 3-isopropylmalate dehydratase, small subunit
  • amino acids 1 to 185 (185 residues), 254 bits, E=4.4e-80
• PF00694: Aconitase_C
  • amino acids 1 to 122 (122 residues), 133.1 bits, E=1.2e-42
• PF27512: LeuD
  • amino acids 127 to 164 (38 residues), 55.3 bits, 6.5e-19
• PF27434: LeuD_C
  • amino acids 176 to 201 (26 residues), 35.6 bits, see alignment (E = 7.3e-13)

Best Hits

Swiss-Prot: 91% identical to LEUD_XANP2: 3-isopropylmalate dehydratase small subunit (leuD) from Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)

KEGG orthology group: K01704, 3-isopropylmalate/(R)-2-methylmalate dehydratase small subunit [EC: 4.2.1.33 4.2.1.35] (inferred from 91% identity to xau:Xaut_1185)

MetaCyc: 51% identical to isopropylmalate isomerase small subunit (Leptospira interrogans serovar Lai str. 56601)
(R)-2-methylmalate dehydratase. [EC: 4.2.1.35]; 3-isopropylmalate dehydratase. [EC: 4.2.1.35, 4.2.1.33]; 4.2.1.33 [EC: 4.2.1.35, 4.2.1.33]; RXN-7744 [EC: 4.2.1.35, 4.2.1.33]

Predicted SEED Role

"3-isopropylmalate dehydratase small subunit (EC 4.2.1.33)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 4.2.1.33)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• L-leucine biosynthesis (6/6 steps found)
• 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
• L-isoleucine biosynthesis II (5/8 steps found)
• 2,5-xylenol and 3,5-xylenol degradation (1/13 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• C5-Branched dibasic acid metabolism
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.33, 4.2.1.35

Use Curated BLAST to search for 4.2.1.33 or 4.2.1.35

Protein Sequence (201 amino acids)

>GFF1843 3-isopropylmalate dehydratase small subunit 1 (Xanthobacter sp. DMC5)
MEKFTVLEGVAAPMKLVNVDTDMIIPKQYLKTIQRTGLGKGLFSEMRYLDDGAENPGFVL
NQPAYRSSKILVAGDNFGCGSSREHAPWALLDFGIRCVISTSFADIFYNNCFKNGILPIV
VSKDDLDKLFDDAERGANATLTIDLPAQEIRGPDGGVVKFEIDPFRKRCLVEGLDDIGLT
LQKAPAIDQYETAKGAEQPWL