Protein Info for GFF1837 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: dTDP-glucose 4,6-dehydratase (EC 4.2.1.46)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01370: Epimerase
  • amino acids 2 to 250 (249 residues), 225.1 bits, E=2.8e-70
• PF02719: Polysacc_synt_2
  • amino acids 2 to 112 (111 residues), 33.7 bits, E=6.9e-12
• PF04321: RmlD_sub_bind
  • amino acids 2 to 176 (175 residues), 44 bits, E=4.6e-15
• TIGR01181: dTDP-glucose 4,6-dehydratase
  • amino acids 2 to 335 (334 residues), 495.6 bits, E=2.7e-153
• PF01073: 3Beta_HSD
  • amino acids 3 to 229 (227 residues), 35.7 bits, E=1.5e-12
• PF16363: GDP_Man_Dehyd
  • amino acids 3 to 323 (321 residues), 310 bits, E=7.6e-96
• PF07993: NAD_binding_4
  • amino acids 4 to 186 (183 residues), 31.1 bits, E=4.2e-11

Best Hits

Swiss-Prot: 70% identical to RMLB_XANCB: dTDP-glucose 4,6-dehydratase (rfbB) from Xanthomonas campestris pv. campestris (strain B100)

KEGG orthology group: K01710, dTDP-glucose 4,6-dehydratase [EC: 4.2.1.46] (inferred from 86% identity to del:DelCs14_5242)

MetaCyc: 61% identical to dTDP-glucose 4,6-dehydratase 2 (Escherichia coli K-12 substr. MG1655)
dTDP-glucose 4,6-dehydratase. [EC: 4.2.1.46]

Predicted SEED Role

"dTDP-glucose 4,6-dehydratase (EC 4.2.1.46)" in subsystem Rhamnose containing glycans or dTDP-rhamnose synthesis or linker unit-arabinogalactan synthesis (EC 4.2.1.46)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• dTDP-β-L-rhamnose biosynthesis (5/5 steps found)
• dTDP-3-acetamido-α-D-fucose biosynthesis (3/5 steps found)
• dTDP-4-O-demethyl-β-L-noviose biosynthesis (3/5 steps found)
• dTDP-β-D-fucofuranose biosynthesis (2/4 steps found)
• dTDP-6-deoxy-α-D-allose biosynthesis (2/4 steps found)
• dTDP-N-acetylthomosamine biosynthesis (2/4 steps found)
• dTDP-N-acetylviosamine biosynthesis (2/4 steps found)
• dTDP-α-D-ravidosamine and dTDP-4-acetyl-α-D-ravidosamine biosynthesis (3/6 steps found)
• dTDP-L-daunosamine biosynthesis (3/6 steps found)
• dTDP-sibirosamine biosynthesis (3/6 steps found)
• dTDP-α-D-mycaminose biosynthesis (2/5 steps found)
• dTDP-3-acetamido-3,6-dideoxy-α-D-glucose biosynthesis (2/5 steps found)
• dTDP-β-L-digitoxose biosynthesis (3/7 steps found)
• dTDP-β-L-olivose biosynthesis (3/7 steps found)
• dTDP-D-desosamine biosynthesis (2/6 steps found)
• dTDP-β-L-4-epi-vancosamine biosynthesis (3/8 steps found)
• dTDP-β-L-megosamine biosynthesis (3/8 steps found)
• dTDP-β-L-mycarose biosynthesis (2/7 steps found)
• dTDP-α-D-forosamine biosynthesis (3/9 steps found)
• dTDP-α-D-olivose, dTDP-α-D-oliose and dTDP-α-D-mycarose biosynthesis (3/9 steps found)
• superpathway of enterobacterial common antigen biosynthesis (2/10 steps found)
• superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis (6/19 steps found)
• superpathway of novobiocin biosynthesis (5/19 steps found)
• superpathway of erythromycin biosynthesis (2/19 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)
• superpathway of megalomicin A biosynthesis (3/22 steps found)
• superpathway of dTDP-glucose-derived antibiotic building blocks biosynthesis (3/23 steps found)

KEGG Metabolic Maps

• Biosynthesis of vancomycin group antibiotics
• Polyketide sugar unit biosynthesis
• Streptomycin biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.46

Use Curated BLAST to search for 4.2.1.46

Protein Sequence (356 amino acids)

>GFF1837 dTDP-glucose 4,6-dehydratase (EC 4.2.1.46) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MILVTGGAGFIGANFVLDWLALHDEPVLNLDKLTYAGNLETLASLQGDARHVFVQGDIGD
PVLLARLLAEHRPRAVVHFAAESHVDRSIHGAEDFIQTNVVGTFRLLEAVRAHWSALPDA
DKAAFRFLHVSTDEVYGSLAPGAPAFTETHTYEPNSPYSASKAASDHLVRAWHHTHGLPV
LTTNCSNNYGPYHFPEKLIPLMIVNALAGKPLPVYGDGLQVRDWLYVKDHCSAIRRVLEA
GALGETYNVGGWNEKPNIEIVHTVCALLDELRPRTDGASYKTQITHVKDRPGHDRRYAID
ARKLEAELGWKPAETFETGIRKTVQWYLDHPDWVAHVQSGTYRQWVQAQYGAPATA