Protein Info for GFF1834 in Xanthobacter sp. DMC5

Annotation: Crotonyl-CoA carboxylase/reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 427 transmembrane" amino acids 324 to 340 (17 residues), see Phobius details TIGR01751: crotonyl-CoA carboxylase/reductase" amino acids 28 to 418 (391 residues), 614.8 bits, see alignment E=2.7e-189 PF08240: ADH_N" amino acids 61 to 176 (116 residues), 59.9 bits, see alignment E=2.6e-20 PF00107: ADH_zinc_N" amino acids 220 to 364 (145 residues), 61.9 bits, see alignment E=6.2e-21

Best Hits

Swiss-Prot: 79% identical to CCR_RHOS4: Crotonyl-CoA reductase (ccr) from Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)

KEGG orthology group: K14446, crotonyl-CoA carboxylase/reductase (inferred from 95% identity to xau:Xaut_1195)

MetaCyc: 84% identical to crotonyl-CoA carboxylase/reductase monomer (Methylorubrum extorquens AM1)
RXN-18384 [EC: 1.3.1.85]; 1.3.1.85 [EC: 1.3.1.85]

Predicted SEED Role

"Crotonyl-CoA carboxylase/reductase, ethylmalonyl-CoA producing"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.1.85

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (427 amino acids)

>GFF1834 Crotonyl-CoA carboxylase/reductase (Xanthobacter sp. DMC5)
MAQTAAANTNEGPVKDLYELGEIPPLGHVPANMYAWTIRRDRHGPPEQSFQLEVVPTWEL
GEQDVLVLVMAAGVNYNGIWAGLGQPISPFDVHKAPFHVAGSDASGIVWAVGSKVKRWKV
GDEVVVHCNQDDGDDEECNGGDPMFSPSQRIWGYETPDGSFGQFCRVQARQLMPRPKHLT
WEESACYTLTMATAYRMLFGHPPHTVKPGDYVLVWGASGGLGVFGVQLAAASGAHVIGVI
SDETKRDYVLGLGAKGVINRKDFKCWGQLPTVNSPEYAEWLKEARKFGKAIWDITGKRDV
DIVFEHPGESTFPVSTLVGKRGGMIVFCAGTTGFNITFDARYVWMRQKRIQGSHFAHLKQ
ASAANQFIIDRRVDPCMSEVFPWDRIPEAHTKMWKNQHAPGNMAVLVNAPRTGLRTFDDV
LEALPKK