Protein Info for GFF1830 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: Two-component sensor protein RcsD (EC 2.7.3.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 889 transmembrane" amino acids 20 to 44 (25 residues), see Phobius details amino acids 309 to 330 (22 residues), see Phobius details amino acids 573 to 588 (16 residues), see Phobius details PF02518: HATPase_c" amino acids 565 to 676 (112 residues), 48.3 bits, see alignment E=1.9e-16 PF16359: RcsD_ABL" amino acids 687 to 788 (102 residues), 147.8 bits, see alignment E=1.5e-47 PF01627: Hpt" amino acids 809 to 883 (75 residues), 49.8 bits, see alignment E=5.1e-17

Best Hits

Swiss-Prot: 84% identical to RCSD_ECOLI: Phosphotransferase RcsD (rcsD) from Escherichia coli (strain K12)

KEGG orthology group: K07676, two-component system, NarL family, sensor histidine kinase RcsD [EC: 2.7.13.3] (inferred from 92% identity to cko:CKO_00554)

Predicted SEED Role

"Two-component sensor protein RcsD (EC 2.7.3.-)" (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (889 amino acids)

>GFF1830 Two-component sensor protein RcsD (EC 2.7.3.-) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MSQSDTTVSTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNAWLKDKSYQIVDITHAI
HKRVDTWRYVTWQIYDNIAATTTPSTGEGLQETRLKQDVYYLEKPRRKTEALIFGSHDSA
TLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLILISTLPLKDLSSGFKESTIGNIVD
SRRAEMLQQANALDERESFSSLRKLAWQNGHYFTLRTTFNQPGHLATVVAFDLPINDLIP
PGMPLDSFRIEPDATQATGRSSEKEAPDSVTISFNGSKIEISSALNSTGMRLIWQVPFGT
LLLDTLQNILLPLLLNIGLLALALFGYATFRHQPGRSTESTSGNAANNELRVLRAINEEI
VSLLPLGLLVYDQEGNRTVISNKIADHLLPHLNLQNITSMAEQHQGVIQATINNELYEIR
LFRSQIAPRTQIFIIRDQDREVLVNKKLKQAQRLYEKNQQGRAAFMQNISNTLKEPVRQL
AVNAAAVTTPESLKLADQADVLVRMIDEIQLANMLENDAWKSEATLFSLQDLIDEVVPEV
LPAIKRKGLQLLINNHLSGNDERWGDRDALRRILLLLIQYAVTTTAMGKITLEVEQDESI
AERLTFRILDTGEGVTLNEIDNLHFPYMNETQGDRYGKANPLTFWLCNQLARKLGGHLNI
KARETLGTRYTVHVKMLPHDQHTQVEERLLDDVSVMVDVTSNEVRAIVLRQLENWGATCI
TPDERQISQEYDLFLTDNPSNLTASGLLLSDDESGVRKIGPGQLRVNFNMSNAMQEAVLQ
LIEEQLAQEEIPASPLGGDENAELHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTA
HRLKGVFAMLNLVPGKQLCETLEHLIREKDAPGIEKYISDIDAYVKSLL