Protein Info for GFF1826 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: Alkylated DNA repair protein AlkB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 216 PF13532: 2OG-FeII_Oxy_2" amino acids 18 to 210 (193 residues), 197.6 bits, see alignment E=1.3e-62

Best Hits

Swiss-Prot: 100% identical to ALKB_SALTY: Alpha-ketoglutarate-dependent dioxygenase AlkB (alkB) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K03919, alkylated DNA repair protein [EC: 1.14.11.-] (inferred from 99% identity to spq:SPAB_00726)

MetaCyc: 80% identical to DNA oxidative demethylase (Escherichia coli K-12 substr. MG1655)
RXN-21233 [EC: 1.14.11.54]; 1.14.11.54 [EC: 1.14.11.54]; RXN-18737 [EC: 1.14.11.54, 1.14.11.33]; 1.14.11.33 [EC: 1.14.11.54, 1.14.11.33]; 1.14.11.33 [EC: 1.14.11.54, 1.14.11.33]; 1.14.11.33 [EC: 1.14.11.54, 1.14.11.33]; 1.14.11.33 [EC: 1.14.11.54, 1.14.11.33]; 1.14.11.- [EC: 1.14.11.54, 1.14.11.33]; RXN0-7275 [EC: 1.14.11.54, 1.14.11.33]

Predicted SEED Role

"Alkylated DNA repair protein AlkB" in subsystem DNA repair, bacterial

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.14.11.-

Use Curated BLAST to search for 1.14.11.- or 1.14.11.33 or 1.14.11.54

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (216 amino acids)

>GFF1826 Alkylated DNA repair protein AlkB (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MLDLFADEAPWQEPLAPGAVVLRRFAFRAAQSLLDDIGFVASQSPFRQMVTPGGYTMSVA
MTNCGALGWTTDRHGYCYAVRDPLTDKPWPALPLSFASVCRQAAIAAGYASFQPDACLIN
RYAPGAKLSLHQDKDEPDLRAPIVSVSLGVPAVFQFGGLRRSDPIQRILLEHGDIVVWGG
ESRLFYHGIQPLKAGFHPMTGEFRYNLTFRQAAEKE