Protein Info for GFF1821 in Variovorax sp. SCN45
Annotation: D-glycerate 2-kinase (EC 2.7.1.165)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 52% identical to TTUD4_AGRVI: Putative hydroxypyruvate reductase (ttuD) from Agrobacterium vitis
KEGG orthology group: K00050, hydroxypyruvate reductase [EC: 1.1.1.81] (inferred from 87% identity to vap:Vapar_3921)Predicted SEED Role
"D-glycerate 2-kinase (EC 2.7.1.-)" in subsystem D-galactarate, D-glucarate and D-glycerate catabolism or Entner-Doudoroff Pathway (EC 2.7.1.-)
MetaCyc Pathways
- L-serine biosynthesis II (3/4 steps found)
- formaldehyde assimilation I (serine pathway) (9/13 steps found)
- photorespiration I (6/9 steps found)
- photorespiration III (6/9 steps found)
KEGG Metabolic Maps
- Glycine, serine and threonine metabolism
- Glyoxylate and dicarboxylate metabolism
- Lipopolysaccharide biosynthesis
- Nicotinate and nicotinamide metabolism
- Pentose phosphate pathway
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.81, 2.7.1.-
Use Curated BLAST to search for 1.1.1.81 or 2.7.1.-
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (446 amino acids)
>GFF1821 D-glycerate 2-kinase (EC 2.7.1.165) (Variovorax sp. SCN45) MTASNPSLAPAPDPREDPRAFLEHLYRVAVDRALPLSTLGPHLPPPPKGRTLVLGCGKAG GSMVQALEALWPADAPLSGLVVTRYDHIPPRPEGLPRRIELVEAAHPVPDEAGLRAAERI LELTKGLTADDLVLCLISGGGSSLLVLPAEGLTLADKQRINTQLLESGASIGEMNCVRKH LSRIKGGRLAAACAPARVVTLTISDVPGDDPAVIASGPTVPDATTCADALAILERYAIEV PAPVRAQLESGALETPKPNDPVFEGHQTHVIATPQQSLEAAAQVVRAAGLEAHILSDEME GESREVGKVHAALARAVALHGQPFARPCVILSGGETTVTIRPRKPGQAKGRGGRGGEFCM GLAGALMGQPGVWALAGDTDGIDGMEDNAGTFVTPDTLARATAKGMKLSDYMDRNDAYTY FGAIGDLLITGPSHTNVNDFRALLVL