Protein Info for GFF1818 in Xanthobacter sp. DMC5

Annotation: D-3-phosphoglycerate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 528 TIGR01327: phosphoglycerate dehydrogenase" amino acids 4 to 526 (523 residues), 689.2 bits, see alignment E=2e-211 PF00389: 2-Hacid_dh" amino acids 5 to 315 (311 residues), 130.5 bits, see alignment E=6.7e-42 PF02826: 2-Hacid_dh_C" amino acids 109 to 283 (175 residues), 209.1 bits, see alignment E=7.1e-66 PF19304: PGDH_inter" amino acids 326 to 444 (119 residues), 158.1 bits, see alignment E=2e-50 PF01842: ACT" amino acids 456 to 518 (63 residues), 36.6 bits, see alignment E=6.1e-13

Best Hits

Swiss-Prot: 43% identical to SERA_METTH: D-3-phosphoglycerate dehydrogenase (serA) from Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)

KEGG orthology group: K00058, D-3-phosphoglycerate dehydrogenase [EC: 1.1.1.95] (inferred from 92% identity to xau:Xaut_1202)

Predicted SEED Role

"D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95)" in subsystem Glycine and Serine Utilization or Pyridoxin (Vitamin B6) Biosynthesis or Serine Biosynthesis (EC 1.1.1.95)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.95

Use Curated BLAST to search for 1.1.1.95

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (528 amino acids)

>GFF1818 D-3-phosphoglycerate dehydrogenase (Xanthobacter sp. DMC5)
MAPRVLISDKLSPAAIQIFKDRGVEVDFQPDLGKDKDKLAEIIGNYDGLAIRSATKVTPK
ILEKANRLKVIGRAGIGVDNVDLPAATAKGVIVMNTPFGNSITTAEHAIAMMFALAREIP
AADASTQAGKWEKNRFMGVEVTGKTLGIIGCGNIGSIVAERGVGLKMKVIAFDPFLSPER
ALDLGVEKVELEDLLARADFITLHTPLTEKTKNILSAENLAKTKKGVRIINCARGGLVDE
EALRAALDSKHVAGAAFDVFTIEPAETNPLFGHPNVVCTPHLGAATTEAQENVALQVAEQ
MSDYLLTGAISNAVNFPSITAEEAPKLKPFIELAEKLGSFAGQLTDTDIKTVRIIYEGAV
ADLKVKALTSAAVAGLLRPALADINVVSAPSVAKERGVVIEETTRAATGDYESLITVTVV
TEKFERSVSGTVFADGHPRIVNIKGIKVDAEFAPAMVYVTNEDKPGFIGRFAGLLGDAGI
NIATFALGRDHAGGDAIALVAVDGAVPAELLAKVQALPQVKAAKALAF