Protein Info for GFF1810 in Xanthobacter sp. DMC5

Annotation: Histidine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 508 TIGR00442: histidine--tRNA ligase" amino acids 11 to 462 (452 residues), 379.3 bits, see alignment E=1.2e-117 PF13393: tRNA-synt_His" amino acids 16 to 188 (173 residues), 86.6 bits, see alignment E=2.8e-28 amino acids 266 to 367 (102 residues), 53.1 bits, see alignment E=4.7e-18 PF00587: tRNA-synt_2b" amino acids 71 to 371 (301 residues), 31 bits, see alignment E=4e-11 PF03129: HGTP_anticodon" amino acids 386 to 463 (78 residues), 37.5 bits, see alignment E=3.3e-13

Best Hits

KEGG orthology group: K01892, histidyl-tRNA synthetase [EC: 6.1.1.21] (inferred from 91% identity to xau:Xaut_1216)

Predicted SEED Role

"Histidyl-tRNA synthetase (EC 6.1.1.21)" (EC 6.1.1.21)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (508 amino acids)

>GFF1810 Histidine--tRNA ligase (Xanthobacter sp. DMC5)
MAKEFKPKARLPRGLVDRTGAELSATRAMLEKIRAVYALYGFEALETPAIEYTDALGKFL
PDLDRPNEGVFSFQDDDEQWLSLRYDLTAPLARYVAEHFEQLPKPYRSYRAGYVFRNEKP
GPGRFRQFMQFDADTVGAPSVAADAEICMMAADTLEAVGIPRGSYVVKVNNRKVLDGVME
AIGLGGDENAGRRLTVLRAIDKFDKFGAEGVALLLGPGRKDESGDFTKGAGLDDLQAAVV
LAMLTGKGVRATDVGVEVELDDVQLAAALAGNAAFQEGMAELNDIRTLLAAGGYQDRVKI
DPSVVRGLEYYTGPVYEAELTFEITDETGRPVRFGSVAGGGRYDGLVARFRGEKVPATGF
SIGVSRLLAALAFLGKMDADAEVGPVVVLAMDKEPARIADYMKMVSELRANGIRAEMYLG
GAGMKAQMKYADRRGAPAVIIQGSNEKDAGEVQIKDLVEGARIAAGIADNAEYRAARPAQ
FAVKESEMIAAIRDVLERRAAPSLGLDD