Protein Info for GFF1795 in Xanthobacter sp. DMC5

Annotation: Bifunctional uridylyltransferase/uridylyl-removing enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 934 TIGR01693: protein-P-II uridylyltransferase" amino acids 63 to 915 (853 residues), 971.8 bits, see alignment E=1.7e-296 PF08335: GlnD_UR_UTase" amino acids 214 to 352 (139 residues), 156.2 bits, see alignment E=2e-49 PF01966: HD" amino acids 501 to 595 (95 residues), 48.9 bits, see alignment E=2.7e-16 PF01842: ACT" amino acids 739 to 795 (57 residues), 29.8 bits, see alignment 1.5e-10 amino acids 848 to 897 (50 residues), 37.6 bits, see alignment 5.3e-13 PF24931: ACT_ACR9_3rd" amino acids 836 to 915 (80 residues), 28.7 bits, see alignment E=4e-10

Best Hits

Swiss-Prot: 64% identical to GLND_BRADU: Bifunctional uridylyltransferase/uridylyl-removing enzyme (glnD) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K00990, [protein-PII] uridylyltransferase [EC: 2.7.7.59] (inferred from 91% identity to xau:Xaut_0284)

Predicted SEED Role

"[Protein-PII] uridylyltransferase (EC 2.7.7.59)" in subsystem Ammonia assimilation (EC 2.7.7.59)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.59

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (934 amino acids)

>GFF1795 Bifunctional uridylyltransferase/uridylyl-removing enzyme (Xanthobacter sp. DMC5)
MPTVRSSKVNRVIGEPFEGQALKAEIREIASAHKGRDLDLRNAVVARLKKGMQEGRAAAE
RLLLEDGCGRCCAMRLSALQDAVITSAYEVATTFLYPVDNPSRSERMAVVAVGGYGRGMM
APGSDTDILFVLPYKQTAWGESVAEAMLYILWDLGLKVGHATRSVDECLRQARADFTIRT
SILEARFLVGDKALFEDLEKRFDKEVVEGTGAEFVAAKMAEREDRLRRSGQSRYLVEPNV
KESKGGLRDLHTLFWISKYVYRVQTASQLVAKGVFTREEANLFKKCEDFLWSVRCHLHFL
TGRAEDRLSFDLQREMALRLGYTEHPGQKDVERFMKHYFLVAKDVGDLTAILSAALEERH
EKPVPGLKGMVERFRAGSRRITLKDTTDFILDHDRLNVADPEAFNRDPVNLIRIFHVADK
RNLALHPDASQLAARSLGLIDASVRENPEANRLFLEIVTSKDAPETVLRRMNEVGVLGRF
LPEFGKIVAMMQFNMYHHYTVDEHLLRCIGILSEIERKVNPENGLANELMGTVKNRNLLY
VALLLHDIAKGRPEDHSVAGARIARKLCPRFGLSPVETETVAWLVEQHLVMSTVAQSRDL
SDRKTIENFASVVQNLERMKLLTILTTADIKAVGPGTWNGWKAQLLRTLYYETEPVLTGG
FSEVDRGKRVTAAQSQFRSALSDWDDEKVAAYVARHYPPYWLRVDLETKLRHARFVDEAE
TAGRSIATHASLEPGRGITTLTVLAPDHPKLLSIIAGACAAAGANIVDAQISTTTDGLAL
DTIAIRRAFDHDEDELRRANRIREAVEQALTGEVRLPEVMAKKLPKSRRTFTVEPEVTVN
NSWSNRHTVVEVSGLDRPGLLFALTNTLSRLNLNIASAHVATFGERAVDVFYVTDLMGAK
ITGAARQSTIRRALVSVFEGPVEDEEPARRVARG