Protein Info for GFF1794 in Xanthobacter sp. DMC5

Annotation: tRNA-specific 2-thiouridylase MnmA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 393 PF00733: Asn_synthase" amino acids 7 to 95 (89 residues), 27.4 bits, see alignment E=8.1e-10 PF03054: tRNA_Me_trans" amino acids 21 to 219 (199 residues), 240.3 bits, see alignment E=4.7e-75 TIGR00420: tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase" amino acids 21 to 379 (359 residues), 311.8 bits, see alignment E=2.5e-97 PF06508: QueC" amino acids 22 to 207 (186 residues), 23.7 bits, see alignment E=9.7e-09 PF20259: tRNA_Me_trans_M" amino acids 230 to 288 (59 residues), 64.1 bits, see alignment E=2e-21 PF20258: tRNA_Me_trans_C" amino acids 296 to 379 (84 residues), 52.8 bits, see alignment E=1.3e-17

Best Hits

Swiss-Prot: 85% identical to MNMA_XANP2: tRNA-specific 2-thiouridylase MnmA (mnmA) from Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)

KEGG orthology group: K00566, tRNA-specific 2-thiouridylase [EC: 2.8.1.-] (inferred from 85% identity to xau:Xaut_0404)

Predicted SEED Role

"tRNA-specific 2-thiouridylase MnmA"

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.-

Use Curated BLAST to search for 2.8.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (393 amino acids)

>GFF1794 tRNA-specific 2-thiouridylase MnmA (Xanthobacter sp. DMC5)
MTDDLLLDASDDARAERAATRVVVAMSGGVDSSVVAALLARAGYDVVGVTLQLYDHGEAA
HRAGSCCAGQDIYDASEVARTLGIPHYVLDYESRFREEVIDRFAASYAAGVTPIPCVDCN
RTVKFRDLLATAEELGARFLATGHYVASRALPGGGRGLFRAAEEARDQSYFLYATTAAQL
DKLRFPLGEMQKADVRALAAEFGLPVAEKPDSQDICFVPGGDYAEVVQRLRPEAGEAGDI
VHLDGRVLGRHKGVLHYTIGQRKGLGIATPEPLYVISIDASRREVRVGPREALAVRAISM
ADVNWLGDIPFAAALDAEAEVYVKVRSTRPPVPARLGRTADGTPAVHLALGEEGVAPGQA
CVLYDAPGAGARLLGGGTIVKAERVAKAVAEVA