Protein Info for GFF1789 in Xanthobacter sp. DMC5

Annotation: Acyl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 389 PF02771: Acyl-CoA_dh_N" amino acids 14 to 125 (112 residues), 119.9 bits, see alignment E=1.9e-38 PF02770: Acyl-CoA_dh_M" amino acids 130 to 223 (94 residues), 82.8 bits, see alignment E=4e-27 PF00441: Acyl-CoA_dh_1" amino acids 235 to 384 (150 residues), 167.1 bits, see alignment E=7.8e-53 PF08028: Acyl-CoA_dh_2" amino acids 252 to 372 (121 residues), 74.6 bits, see alignment E=2.4e-24 PF22924: ACOX_C_alpha1" amino acids 280 to 385 (106 residues), 33.4 bits, see alignment E=9.6e-12

Best Hits

Swiss-Prot: 59% identical to ACAD8_HUMAN: Isobutyryl-CoA dehydrogenase, mitochondrial (ACAD8) from Homo sapiens

KEGG orthology group: None (inferred from 87% identity to xau:Xaut_2190)

MetaCyc: 42% identical to short-chain acyl-CoA dehydrogenase monomer (Pseudomonas putida KT2440)
RXN-13449 [EC: 1.3.8.1]

Predicted SEED Role

"Branched-chain acyl-CoA dehydrogenase (EC 1.3.99.12)" in subsystem Isobutyryl-CoA to Propionyl-CoA Module or Isoleucine degradation or Leucine Degradation and HMG-CoA Metabolism or Valine degradation (EC 1.3.99.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.8.1 or 1.3.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (389 amino acids)

>GFF1789 Acyl-CoA dehydrogenase (Xanthobacter sp. DMC5)
MARTKAPPNMFTLSEDQIAIRDMARDFANEQIAPYAVEWDEKKHFPVEQLRAAAALGMGG
IYVKEDVGGSGLTRLDAALIFEQLATGCPGTSAYLSIHNMSTWMIDRFGDDAQRRKWVPR
LASMEHFASYCLTEPGAGSDAAALKTKAVRNGDHYIVDGQKQFISGAGSSDIYVVMVRTG
GDGPKGISTLVIEAGAPGLSFGANERKMGWNVQPTRTVTFEGVRVPVENRLGEEGEGFRI
AMAGLDGGRLNIGACSLGGAQAALEKALSYMRERKAFGKRLDEFQALQFRLADMATELEA
ARTFLWRAASALDAKAPDATQLCAMAKRIATDRGFEVANDALQMHGGYGYLADYGIEKIV
RDLRVHQILEGTNEIMRLIVARALIAQGN