Protein Info for GFF1768 in Xanthobacter sp. DMC5

Annotation: Threonylcarbamoyladenosine tRNA methylthiotransferase MtaB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 428 TIGR00089: radical SAM methylthiotransferase, MiaB/RimO family" amino acids 12 to 426 (415 residues), 310.4 bits, see alignment E=1.9e-96 PF00919: UPF0004" amino acids 13 to 97 (85 residues), 49.1 bits, see alignment E=4.7e-17 TIGR01579: MiaB-like tRNA modifying enzyme" amino acids 14 to 404 (391 residues), 406.6 bits, see alignment E=1.5e-125 PF04055: Radical_SAM" amino acids 151 to 323 (173 residues), 78.8 bits, see alignment E=5.6e-26

Best Hits

KEGG orthology group: None (inferred from 85% identity to xau:Xaut_0412)

Predicted SEED Role

"tRNA-t(6)A37 methylthiotransferase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (428 amino acids)

>GFF1768 Threonylcarbamoyladenosine tRNA methylthiotransferase MtaB (Xanthobacter sp. DMC5)
MAEGPGVQRGVDVVNFGCRLNALEGDGIARAATAAGLERALVVNTCAVTAEAVRQARQAI
RRARRRDPALRVIVTGCAAQTEPATFAGMGEVDLVLGNAAKTSAETWAGARTSLDFGVSA
EQKVQVQDISAVREATPHLADRFEGHTRAFVEVQNGCDHSCTFCIIPFGRGPSRSVPMGA
VVAQVARLVESGHGEVVLTGVDLTAYGADLPGAPTLGRLVRAVLSGVPELKRLRLSSIDA
VEADAELMRALAEEERLMPHLHLSLQSGDDLILKRMKRRHSRAEALAFIAALRQARPDVV
LGADIIAGFPTETEAQARATRDFVEEAGLAFLHAFPYSARPGTAAARMPQLAPALVAERA
ARLRETGAGLLRRHLSAEVGRRRTVLVEAGGRGHTEHFTPVRLSGAAVRGSLADLRIAGH
DGARLIAA