Protein Info for GFF176 in Sphingobium sp. HT1-2

Annotation: Cytosol aminopeptidase PepA (EC 3.4.11.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 481 PF02789: Peptidase_M17_N" amino acids 34 to 130 (97 residues), 37.3 bits, see alignment E=2.5e-13 PF00883: Peptidase_M17" amino acids 165 to 471 (307 residues), 414 bits, see alignment E=4.2e-128

Best Hits

Swiss-Prot: 55% identical to AMPA_CAUVC: Probable cytosol aminopeptidase (pepA) from Caulobacter vibrioides (strain ATCC 19089 / CB15)

KEGG orthology group: K01255, leucyl aminopeptidase [EC: 3.4.11.1] (inferred from 81% identity to sjp:SJA_C1-25520)

Predicted SEED Role

"Cytosol aminopeptidase PepA (EC 3.4.11.1)" (EC 3.4.11.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.11.1

Use Curated BLAST to search for 3.4.11.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (481 amino acids)

>GFF176 Cytosol aminopeptidase PepA (EC 3.4.11.1) (Sphingobium sp. HT1-2)
MDIQFSPTRPQVDTLVLAVAKGTVDSLPLTASTTLVAGAAAARFAGEAGGSVESFVEEGG
AVLRVLLLGIGNGSAADLERAGGALTAKLSTSGATHAAVEFPAGVSGEDAARLGLGALLR
SWRIDTYRTRQPEKAKPTLATVTIVANGAELEWAKLSAVAAGVAFTRELVSEPANILYPE
SFVERCQHLAELGVKITVLDRAAMTDLGMGALLGVAQGSAREARLLAMEWDGTDGAADKP
VVFIGKGVTFDTGGISLKPGPGMEDMKWDMGGAGAVAGAMKALAGRKAKARVVGICGLVE
NMPDANAQRPGDIVTSMSGQTIEVLNTDAEGRLVLCDAVTWAQKTYEPEVVIDLATLTGA
MMIALGSEHGGLFANDDALADDLLAAGKTSGEKLWRFPLSEAYDKLIDSPIADMKNIGPR
FAGSITAAQFIKRFVNEGVKWAHLDIAGMVWSDKPGATADKGATGYGVRLIDQLVADKFE
R