Protein Info for GFF1759 in Sphingobium sp. HT1-2

Annotation: alpha-galactosidase (EC 3.2.1.22)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF16499: Melibiase_2
  • amino acids 189 to 363 (175 residues), 94.5 bits, E=7.4e-31
• PF17801: Melibiase_C
  • amino acids 377 to 449 (73 residues), 51.6 bits, E=8.3e-18

Best Hits

KEGG orthology group: None (inferred from 68% identity to cse:Cseg_1839)

Predicted SEED Role

"Alpha-galactosidase precursor (EC 3.2.1.22)" in subsystem Galactosylceramide and Sulfatide metabolism (EC 3.2.1.22)

MetaCyc Pathways

• melibiose degradation (1/1 steps found)
• stachyose degradation (3/7 steps found)

KEGG Metabolic Maps

• Galactose metabolism
• Glycerolipid metabolism
• Glycosphingolipid biosynthesis - globo series
• Sphingolipid metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.22

Protein Sequence (452 amino acids)

>GFF1759 alpha-galactosidase (EC 3.2.1.22) (Sphingobium sp. HT1-2)
MTWKASRRDLFAGGVAGLAAAGAVRAGAVPAEAGKAVLAPRPPMGWNSWNSFATTITEAQ
ARETAGIMAEKLLPFGYDIFTVDIQWYEPEASSYTYNAKPRPAMDGYGRMIPAPNRFPSS
VGGKGFTQLAKEVHALGMKFGIHVMRGIPRAAVEQNLPILGTKYRAADIADRKSICSWNP
DMYGVDMTRPGAQAYYDSIFRLYADWGVDFVKMDDMSRPYDAHAAEIEAAHKAIVATGRP
IILSLSPGETPVMRGDHVRKYAQMWRISDDFWDDWAMLEAQFTRLENWTPYRGPGSWPDA
DMLPLGRLALGERDTRFTPDEQRTLMTLWAIARSPLIMGGDLRHLDAATLALLTNKEVIA
VNQASHDNQPHYIEDGGRIWSAKAEGSADRYVALFNVSDKPREVWIRLRDLGLNGPVAVR
DLWAGQALGQQADRISVTLPPHGAGLYRLSAA