Protein Info for GFF1739 in Variovorax sp. SCN45

Annotation: Glutamate carboxypeptidase (EC 3.4.17.11)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 425 signal peptide" amino acids 1 to 40 (40 residues), see Phobius details PF04389: Peptidase_M28" amino acids 107 to 214 (108 residues), 38.3 bits, see alignment E=2.5e-13 PF01546: Peptidase_M20" amino acids 122 to 419 (298 residues), 113.9 bits, see alignment E=2e-36 PF07687: M20_dimer" amino acids 223 to 323 (101 residues), 74.3 bits, see alignment E=1.5e-24

Best Hits

KEGG orthology group: K01295, glutamate carboxypeptidase [EC: 3.4.17.11] (inferred from 90% identity to vpe:Varpa_4614)

Predicted SEED Role

"Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases"

Isozymes

Compare fitness of predicted isozymes for: 3.4.17.11

Use Curated BLAST to search for 3.4.17.11

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (425 amino acids)

>GFF1739 Glutamate carboxypeptidase (EC 3.4.17.11) (Variovorax sp. SCN45)
MISSKRSSSKFTRNPAPLRALCHGVVLACALLAGGNALAQKPHQGALDAATQYKDEALKM
LERMVNIDSGSANTEGLDKVREMAVGELRKLGARIETFPADPHPGTNVVATFTGQGKKKI
LILAHMDTVFKDGTAAAKPFYIQDGRAYGPGVMDDKGGVVAGLYALKILQQIGFKDYGQI
TFLLDTNEETGSVGTSALIERIAKEHDVALNLEPGRPADGLVVERKGSATALVEVKGVAA
HAGVAPEAGRNAAMEVAHQVLQLSKTADPVKKTTVNFTVLTANGPTNVIPATASAKGDVR
AATPEEFDRVEKDLVRIAQNKLIPETEVRVRLVRGLPPMPRLPASEKLVKMAEGIYAEIG
KKLTIESSGGAADASLVAGVGVPVLDGFGIVGGGIHTPEEYAEVESVVPRLYLLSRMLMD
LSANN