Protein Info for GFF1718 in Xanthobacter sp. DMC5

Annotation: Efflux pump periplasmic linker BepF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 408 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 48 to 384 (337 residues), 289.2 bits, see alignment E=1.7e-90 PF13533: Biotin_lipoyl_2" amino acids 74 to 121 (48 residues), 48.6 bits, see alignment 8e-17 PF16576: HlyD_D23" amino acids 74 to 303 (230 residues), 51.7 bits, see alignment E=1.1e-17 PF13437: HlyD_3" amino acids 187 to 300 (114 residues), 34.4 bits, see alignment E=4.7e-12

Best Hits

KEGG orthology group: None (inferred from 80% identity to xau:Xaut_2359)

Predicted SEED Role

"Probable Co/Zn/Cd efflux system membrane fusion protein" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (408 amino acids)

>GFF1718 Efflux pump periplasmic linker BepF (Xanthobacter sp. DMC5)
MSPAPRLSVASHFASAAAAVPVLALALALAGCGQPTAQQQGAPPPPQVTVANPTSANVTD
TDEYVGRFVAIDEVNVRARVSGYLDKIDFVDGQLVKEGDVLFTIDQRPFKIAVDQAQANL
AQAKANLDFTKADLERARTLLEDKTSNAISKQAFDQRTQAERTAAAIVQSQEAQVRSAQL
DLGFTEVKAPVSGQIGDRRVSVGNYVTGGTGGTPTLLAVIVSTDPIRFEFTIDEASLLRL
TRRKGGTDFKGEPVTLKLIDDKDFVHKGKLDFLNNVVDRETGTIRGRAIFDNKTGLFRPG
MFARIRLQSSDPYQALVVPDVAIGTEQVKKFVYVVGPDNKVSQKFVTLGPLQGDMRVIRE
GLGPDDKVVVNGLMRVRPGVTVTPQVAGAAPAAGAPGAPAGSSSAASK