Protein Info for PS417_08740 in Pseudomonas simiae WCS417

Annotation: ribose-phosphate pyrophosphokinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02322: phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN
  • amino acids 3 to 183 (181 residues), 220.5 bits, E=6.6e-70

Best Hits

Swiss-Prot: 95% identical to PHNN_PSEFS: Ribose 1,5-bisphosphate phosphokinase PhnN (phnN) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K05774, ribose 1,5-bisphosphokinase [EC: 2.7.4.23] (inferred from 95% identity to pfs:PFLU1788)

Predicted SEED Role

"ATP-binding protein PhnN; Guanylate kinase (EC 2.7.4.8)" (EC 2.7.4.8)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (24/26 steps found)
• superpathway of purine nucleotide salvage (13/14 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis I (6/6 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis II (7/8 steps found)
• guanosine ribonucleotides de novo biosynthesis (4/4 steps found)
• dZTP biosynthesis (3/5 steps found)
• glyphosate degradation III (3/7 steps found)
• methylphosphonate degradation I (1/5 steps found)
• (aminomethyl)phosphonate degradation (3/8 steps found)

KEGG Metabolic Maps

• Pentose phosphate pathway
• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.4.8

Use Curated BLAST to search for 2.7.4.23 or 2.7.4.8

Sequence Analysis Tools

See U1ULB2 at UniProt or InterPro

Protein Sequence (190 amino acids)

>PS417_08740 ribose-phosphate pyrophosphokinase (Pseudomonas simiae WCS417)
MAGRLIYLIGPSGSGKDSLLDAARPRLAERGCRIVRRVITRSAEAVGEAAQGVSPEQFAS
MQAEGAFALSWQANGLSYGIPKVIDDWLAAGDDVLVNGSRAHLAQTRERYPTSLVLLLTV
DQAVLRQRLIARGREALVDIDARLARNARFTAELIAGNGAGLFVLDNSGPLEHTVERLLC
CLDHGHSACA