Protein Info for GFF1709 in Xanthobacter sp. DMC5

Annotation: 4-hydroxyphenylacetate 3-monooxygenase oxygenase component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 484 transmembrane" amino acids 289 to 308 (20 residues), see Phobius details PF11794: HpaB_N" amino acids 4 to 267 (264 residues), 279 bits, see alignment E=4.9e-87 PF03241: HpaB" amino acids 279 to 474 (196 residues), 178.4 bits, see alignment E=1e-56

Best Hits

KEGG orthology group: K00483, 4-hydroxyphenylacetate-3-hydroxylase large chain [EC: 1.14.13.3] (inferred from 96% identity to xau:Xaut_2574)

Predicted SEED Role

"4-hydroxyphenylacetate 3-monooxygenase (EC 1.14.13.3)" in subsystem 4-Hydroxyphenylacetic acid catabolic pathway or Aromatic Amin Catabolism (EC 1.14.13.3)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.14.13.3

Use Curated BLAST to search for 1.14.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (484 amino acids)

>GFF1709 4-hydroxyphenylacetate 3-monooxygenase oxygenase component (Xanthobacter sp. DMC5)
MIRTGAAYKEGLRDGREIWIDGERVADVTTHPAFKPIVDIKARMYDLHHEEATAPALTYV
EENAVHSVFHQPPTETAHWDAKRKGVDILLKDVGGVVTRVGDETIGEMWSLQDGRDMLAA
IDPRFAANIDNHIRRVVEEDPFHVSANTDPKGDRSRHISERDPDMMVHVTKETDAGIIIR
GAKYETAAAYADQAYLKPTVGAWTDEKESDYALGCIVKMGAPGVKHICRSSFAGRSNPKD
YPLANRYDEVDTLVVFDDVLIPWEDVFFYRYTKAAQFVRGTLHRYSAYPYVLRLLYVADM
MIGAALWNAKQTGLDKLQSVQEKLADLAVYREGINAHLTASIALAEKSPAGLLMPNQSIL
YAGRVLACSQLPTMMHATRELIGGQICVTPNVAAFEAEGTAEWLKKFYTLNDNWESEDRR
KLLAFARDLVSSDYAGHRLAFVQFAQAPHFNHLAAVYKSFDFSGPLDFVRKTAGLSDNVM
GSLK