Protein Info for GFF1699 in Xanthobacter sp. DMC5

Annotation: Arginine biosynthesis bifunctional protein ArgJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00120: glutamate N-acetyltransferase/amino-acid acetyltransferase
  • amino acids 19 to 413 (395 residues), 372.8 bits, E=9.8e-116
• PF01960: ArgJ
  • amino acids 24 to 413 (390 residues), 467.2 bits, E=1.7e-144

Best Hits

Swiss-Prot: 72% identical to ARGJ_BRADU: Arginine biosynthesis bifunctional protein ArgJ (argJ) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K00620, glutamate N-acetyltransferase / amino-acid N-acetyltransferase [EC: 2.3.1.1 2.3.1.35] (inferred from 89% identity to xau:Xaut_2584)

Predicted SEED Role

"Glutamate N-acetyltransferase (EC 2.3.1.35) / N-acetylglutamate synthase (EC 2.3.1.1)" in subsystem Arginine Biosynthesis extended (EC 2.3.1.1, EC 2.3.1.35)

MetaCyc Pathways

• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• superpathway of arginine and polyamine biosynthesis (14/17 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• L-ornithine biosynthesis I (5/5 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.1 or 2.3.1.35

Protein Sequence (413 amino acids)

>GFF1699 Arginine biosynthesis bifunctional protein ArgJ (Xanthobacter sp. DMC5)
MSTTVSPLAPKSVPELKPVPGVTFTTVAAGIRYKGRTDVLAMAFEPGTTVAGVTTRSLCP
SAPVEWCRELLKKGKARALVVNSGNANAFTGMKGREATSLTADLAAQAFGCKPTEVFLAS
TGVIGEPLDANKFEAALTEAAGQLVDWPWLDAARAIMTTDTFPKLATATVLLGDVPVTIN
GIAKGAGMIAPDMATMLSFVFTDAPIAADVLQKLLSKGVKTTFNAVTIDGDTSTSDTLLL
FATGAAKERGAKTVKDIDDPALKAFKRALIELLADLAEQVARDGEGARKLVRITVTGAVS
NKSARRIAMSIANSPLVKTAVAGEDANWGRVVMAVGKAGEPADRDLLSISFGDIRVAHEG
ARDPDYDEAAVSDYMKNDVIDLTVDLGLGRGTDRVLTCDLTKEYVAINGDYRS