Protein Info for GFF1693 in Variovorax sp. SCN45

Annotation: Acetoacetyl-CoA reductase (EC 1.1.1.36)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01829: acetoacetyl-CoA reductase
  • amino acids 6 to 248 (243 residues), 355.6 bits, E=7.1e-111
• PF00106: adh_short
  • amino acids 6 to 198 (193 residues), 205 bits, E=1.3e-64
• PF08659: KR
  • amino acids 8 to 182 (175 residues), 97.9 bits, E=1.1e-31
• PF13561: adh_short_C2
  • amino acids 16 to 247 (232 residues), 202.5 bits, E=1.2e-63

Best Hits

Swiss-Prot: 55% identical to PHAB_CUPNH: Acetoacetyl-CoA reductase (phaB) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K00023, acetoacetyl-CoA reductase [EC: 1.1.1.36] (inferred from 96% identity to vap:Vapar_5342)

MetaCyc: 55% identical to hydroxyvaleryl-CoA reductase / acetoacetyl-CoA reductase (Cupriavidus necator)
Acetoacetyl-CoA reductase. [EC: 1.1.1.36]; 1.1.1.36 [EC: 1.1.1.36]

Predicted SEED Role

"Acetoacetyl-CoA reductase (EC 1.1.1.36)" in subsystem Acetyl-CoA fermentation to Butyrate or Polyhydroxybutyrate metabolism or Serine-glyoxylate cycle (EC 1.1.1.36)

MetaCyc Pathways

• acetyl-CoA fermentation to butanoate (6/7 steps found)
• polyhydroxybutanoate biosynthesis (3/3 steps found)
• (2S)-ethylmalonyl-CoA biosynthesis (3/4 steps found)
• (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) (3/5 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (18/26 steps found)
• 4-oxopentanoate degradation (3/9 steps found)
• ethylmalonyl-CoA pathway (4/11 steps found)

KEGG Metabolic Maps

• Butanoate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.36

Use Curated BLAST to search for 1.1.1.36

Protein Sequence (249 amino acids)

>GFF1693 Acetoacetyl-CoA reductase (EC 1.1.1.36) (Variovorax sp. SCN45)
MSEKQRVVLVTGGMGGLGETISTKMVDAGYKLAVTYSPGNKSHADWVAQMAERGYRILAV
PCDVADYDACAQAVSQVQEALGPVDVLVNNAGITRDMTFRKMDQINWNAVLRTNLDSLFN
MSKQVADGMVERGWGRIINVSSVNGSKGAFGQTNYSAAKAGVHGFTKALALEVARKGVTV
NTISPGYIGTKMVTAIPKEVLDSKILPHIPVGRLGRPEEVAGLIIYLASQEAAFVTGANI
AINGGQHMQ