Protein Info for PS417_08595 in Pseudomonas simiae WCS417

Annotation: histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1215 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details transmembrane" amino acids 544 to 565 (22 residues), see Phobius details PF00497: SBP_bac_3" amino acids 71 to 281 (211 residues), 43.1 bits, see alignment E=1.4e-14 amino acids 311 to 529 (219 residues), 71.3 bits, see alignment E=2.7e-23 TIGR00229: PAS domain S-box protein" amino acids 585 to 703 (119 residues), 31 bits, see alignment E=1.2e-11 PF00989: PAS" amino acids 585 to 697 (113 residues), 33.8 bits, see alignment E=1.2e-11 PF08448: PAS_4" amino acids 592 to 702 (111 residues), 41.4 bits, see alignment E=5.9e-14 PF00512: HisKA" amino acids 720 to 786 (67 residues), 62.1 bits, see alignment 1.7e-20 PF02518: HATPase_c" amino acids 835 to 949 (115 residues), 90 bits, see alignment E=5.6e-29 PF00072: Response_reg" amino acids 974 to 1084 (111 residues), 77.9 bits, see alignment E=2.7e-25 PF01627: Hpt" amino acids 1118 to 1201 (84 residues), 39.2 bits, see alignment 2.8e-13

Best Hits

KEGG orthology group: K07679, two-component system, NarL family, sensor histidine kinase EvgS [EC: 2.7.13.3] (inferred from 85% identity to pfs:PFLU1739)

Predicted SEED Role

"Hybrid sensory histidine kinase in two-component regulatory system with EvgA" in subsystem Orphan regulatory proteins

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7TZN8 at UniProt or InterPro

Protein Sequence (1215 amino acids)

>PS417_08595 histidine kinase (Pseudomonas simiae WCS417)
MRKRMPKCIKDYLIILSAGVCLSTTVLAAATQPEHYNLLSRAGNVQLDTPMDATQRRWLQ
NKRELILGTAAPDYPPFDITSSGYDYEGITADYAGVLAKALALPVRVQRYPTREASIQAL
ESGEIDLLGSSNGFEATHPLLTLSAPYAVDQPVLVTREGETRSLSDGLAGLRLSLVYHYL
PLEEVQKLYPKAIIRAYPSYQNALNAVAFDQADVFLGDTISTHYMINKGYLKNIHMANFG
KHEAYGFSFALRQDQPILLNIVNAVLAALPTHERENIAKRWSAGSDILLTDQKLQLTQRE
ERWLKAHPVVTVIANETFAPLTFFDADGNFRGITADLLELIRLRTGLRFEIQRGRDINAM
IEQIDTGKVDIIGAIVPSSERETQLNFSRPYLENSYVLLTRKEPDAPLNLEQMAGKRLAI
TQGNPLEVTLRKDFPHIQLVETSDTFKAAELLAQGHVEGAVNSLVVANYLLSSHVFQDRL
QIGTSIGTLPALFALATSRDATELSSILDKALLSIAPDELGVINSRWRGYTAASDSYWRD
YNDLIAQIIIGTGLLLLISLAWNAYMRRQIQHRRMAERALNDQFEFMRALVNETPHPIYV
RDRNGLLQTCNDSYLQVFDVKREDVIGKSALQISTALETEASQYHADYQRVVAQGNPLIV
DRPLHIRGKKLTIYHWILPYRDSTGEVQGIIGGWIDISERRQLFDELRAAKERADEANRA
KSTFLATMSHEIRTPMNAVIGMLELTLKRADQGHLDRPAIEVAYHSAKDLLELIGDILDI
ARIESGRLSLSPERVNLKDVIESVVRVFDGLARQKTLSLLLEFKPGLDDTDVLIDPLRFK
QVLSNLVSNAIKFTERGEVKVKVEVHATDLPQQVEMKLVVEDTGIGISREDQLRLFEPFS
QADNSGHLARSGAGLGLVICRSLCAMMGGQLSLSSVPMVGTQVYVSLKMHSLQPVHAVEE
PKPATPTPAPVLNVLVVDDHPANRLLMCQQLGYLGHQFTAAQHGAAGFQAWRQEHFDLVI
ADCNMPIMNGYELSRSIREYEQREQLPPCVILGFTANAQPEEKRRCAEAGMNDCLFKPIS
LTILERQLAQIGPHPTSTLLDLSSLDALTGGDPQLSRRLLEELLSSSAHDRQEIIALVAR
QAPLQDIIEQAHKIKGAARIVQASVLAAQCEALEQGCARGDELPLIETGIKTLEKCMLEL
ERQLRMQLDSLTTEQ