Protein Info for GFF1674 in Sphingobium sp. HT1-2

Annotation: ATP-dependent protease La (EC 3.4.21.53) Type I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 799 PF02190: LON_substr_bdg" amino acids 5 to 195 (191 residues), 173.9 bits, see alignment E=1.2e-54 TIGR00763: endopeptidase La" amino acids 6 to 766 (761 residues), 958.6 bits, see alignment E=1.6e-292 PF07728: AAA_5" amino acids 348 to 481 (134 residues), 33.4 bits, see alignment E=1.2e-11 PF00004: AAA" amino acids 348 to 485 (138 residues), 74.5 bits, see alignment E=3.5e-24 PF22667: Lon_lid" amino acids 509 to 559 (51 residues), 71.2 bits, see alignment 1.4e-23 PF05362: Lon_C" amino acids 565 to 767 (203 residues), 310.7 bits, see alignment E=1.3e-96

Best Hits

Swiss-Prot: 67% identical to LON_AZOBR: Lon protease (lon) from Azospirillum brasilense

KEGG orthology group: K01338, ATP-dependent Lon protease [EC: 3.4.21.53] (inferred from 93% identity to sch:Sphch_0637)

MetaCyc: 61% identical to Lon protease (Escherichia coli K-12 substr. MG1655)
Endopeptidase La. [EC: 3.4.21.53]

Predicted SEED Role

"ATP-dependent protease La (EC 3.4.21.53) Type I" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent (EC 3.4.21.53)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.21.53

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (799 amino acids)

>GFF1674 ATP-dependent protease La (EC 3.4.21.53) Type I (Sphingobium sp. HT1-2)
MTTQYPLLPLRDIVVFPQMIVPLFVGRDKSVAALEAAMEGNKEIFLVSQLDPAEDDPGKD
ALYDTGVVSVVLQLLKLPDGTVRVLVEGKHRAQLADLAPAEAGYLVAEVAPVEEIVAEGP
EAAALMRSVAEQFENYAKLNKKLPAETPVQLREIDDAGRLADSVAANINVKVADKQSLLV
EPDPVKRLEMVFAFMEGELGVLQVEKKIRGRVKRQMEKTQREYYLNEQLKAIQRELGNGE
GEEGDELAELADKIAKAKLSKEARAKATAELKKLKGMQPMSAEATVVRNYLDVLLGLPWG
KKGRVKTDLKKAQAILDEDHFALEKVKDRIIEYLAVQARTNKLKGPILCLVGPPGVGKTS
LGRSIAKATGREFVRQSLGGVRDEAEIRGHRRTYIGSLPGKVVSNLKKAGTMNPLFLLDE
IDKLGQDFRGDPASALLEVLDPEQNSKFQDHYLEIDVDLSDVMFVTTANSLNLPQPLLDR
MEIIRLEGYTEDEKVEIAQRHLVPKQIDAHGLKNGEFEVTEAAVRDLIRYYTREAGVRTL
EREVARLARKALRKILEGAFDKVEITPENLADYAGVRKFRHGVGEEENQIGAVTGLAWTE
VGGELLTIEAVTVPGKGLIKTTGKLGEVMNESVQAAFSYVKARSPGYGIKPSLFNRKDIH
IHLPEGAVPKDGPSAGIGMVTTIVSTLTGIPVHKDVAMTGEVTLRGRVLPIGGLKEKLLA
ALRGGIKTVLIPQENEKDLAEIPANIREGLEIVPVSHVDEVLARALVSKPEAITWTEEDD
LAAQPSAGQGRDGDPAIRH