Protein Info for GFF1670 in Variovorax sp. SCN45

Annotation: 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02087: 3-isopropylmalate dehydratase, small subunit
  • amino acids 13 to 164 (152 residues), 155 bits, E=6.6e-50
• PF00694: Aconitase_C
  • amino acids 57 to 113 (57 residues), 57 bits, E=1.3e-19

Best Hits

Swiss-Prot: 40% identical to HACB1_METAC: Methanogen homoaconitase small subunit 1 (hacB1) from Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)

KEGG orthology group: K01704, 3-isopropylmalate/(R)-2-methylmalate dehydratase small subunit [EC: 4.2.1.33 4.2.1.35] (inferred from 85% identity to vap:Vapar_0799)

MetaCyc: 42% identical to homoaconitase small subunit (Thermus thermophilus)
Methanogen homoaconitase. [EC: 4.2.1.114, 4.2.1.36]

Predicted SEED Role

"3-isopropylmalate dehydratase small subunit (EC 4.2.1.33)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 4.2.1.33)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• L-leucine biosynthesis (6/6 steps found)
• 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
• L-isoleucine biosynthesis II (5/8 steps found)
• 2,5-xylenol and 3,5-xylenol degradation (5/13 steps found)
• L-lysine biosynthesis IV (2/9 steps found)
• L-lysine biosynthesis V (2/10 steps found)
• coenzyme B biosynthesis (2/16 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• C5-Branched dibasic acid metabolism
• Lysine biosynthesis
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.33, 4.2.1.35

Use Curated BLAST to search for 4.2.1.114 or 4.2.1.33 or 4.2.1.35 or 4.2.1.36

Protein Sequence (175 amino acids)

>GFF1670 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33) (Variovorax sp. SCN45)
MNMLTTTGRPVHRVWRVGADIDTDALAPGHAMKHGVDVIAKHCLEAIRPEFAAQVRPGDV
IVAGPNFGIGSSREQAAAVLVQLGVAAVIAPSYSGLYFRNAFNVGLLLLTCAEAETLEEG
ESITLDTRTPALHTAGARALACEPVPDFLMEMVDAGGLLRLLRQRMAARKEVDAH