Protein Info for PS417_08485 in Pseudomonas simiae WCS417

Annotation: alpha/beta hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 338 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF07224: Chlorophyllase" amino acids 80 to 208 (129 residues), 26.3 bits, see alignment E=4.8e-10 PF20434: BD-FAE" amino acids 89 to 197 (109 residues), 59.7 bits, see alignment E=4.5e-20 PF07859: Abhydrolase_3" amino acids 104 to 310 (207 residues), 241.5 bits, see alignment E=1.2e-75

Best Hits

KEGG orthology group: None (inferred from 96% identity to pfs:PFLU1715)

MetaCyc: 40% identical to tert-butyl formate deformylase (Methylibium petroleiphilum PM1)
3.7.1.-

Predicted SEED Role

"Lipase"

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7TXG9 at UniProt or InterPro

Protein Sequence (338 amino acids)

>PS417_08485 alpha/beta hydrolase (Pseudomonas simiae WCS417)
MNTIGKALTGTLLALSITNAFAASGEVEHSTQAFLDVLNAGTGKPMEQLTPKDARAVLTG
AQAGVKLTLPKADVSQKTIQVDGKPLDLTIVRPAGVKGTLPVFMFFHGGGWVLGDYPTHE
RLVRDLVVGSGAVAVFVNYTPSPEAHYPVAINQAYGATKWVAEHGQEINVDGKRLAVAGN
SVGGNMAAVVSLMAKDKGTPAIKFQVLLWPVTDANFDTGSYNQYAEGHFLTRNMMKWFWD
NYTIDANQRAEIYASPLRATLDQLKGLPPALIQTAGADVLRDEGEAYARKLDAAGVAVTA
VRYNGMIHDYGLLNVVSQVPAVRSALLQASDELKQHLK