Protein Info for GFF1661 in Xanthobacter sp. DMC5

Annotation: Release factor glutamine methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 312 PF17827: PrmC_N" amino acids 17 to 86 (70 residues), 64.5 bits, see alignment E=4.2e-21 TIGR00536: methyltransferase, HemK family" amino acids 19 to 293 (275 residues), 186.5 bits, see alignment E=5.6e-59 TIGR03534: protein-(glutamine-N5) methyltransferase, release factor-specific" amino acids 36 to 288 (253 residues), 249.2 bits, see alignment E=4.5e-78 PF03602: Cons_hypoth95" amino acids 105 to 205 (101 residues), 27 bits, see alignment E=1.3e-09 PF05175: MTS" amino acids 125 to 208 (84 residues), 48.6 bits, see alignment E=2.9e-16 PF13847: Methyltransf_31" amino acids 126 to 253 (128 residues), 37.8 bits, see alignment E=6.2e-13 PF06325: PrmA" amino acids 127 to 198 (72 residues), 23.6 bits, see alignment E=1.4e-08 PF13649: Methyltransf_25" amino acids 128 to 202 (75 residues), 33.1 bits, see alignment E=3.1e-11

Best Hits

Swiss-Prot: 48% identical to PRMC_RHORT: Release factor glutamine methyltransferase (prmC) from Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)

KEGG orthology group: K02493, methyltransferase [EC: 2.1.1.-] (inferred from 73% identity to xau:Xaut_2622)

Predicted SEED Role

"Protein-N(5)-glutamine methyltransferase PrmC, methylates polypeptide chain release factors RF1 and RF2"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (312 amino acids)

>GFF1661 Release factor glutamine methyltransferase (Xanthobacter sp. DMC5)
VPGPGDTPAGDLTIARLRRQMTARLAAAGVETPELDARLLLAHALGCAPGELLERGTTSA
TPGMSATAEALLARRLAGEPVARIFGHKEFWSLTFRLSPDTLVPRPDTETVVEAALSLFP
DRTAPLRILDLGTGTGAILAALLAERPAATGIAVDRSENAARTALANLEANGLADRASVV
VGDWGNALVGGFDLVVSNPPYIAEDEMDGLAVDVRFHDPHLALVAGADGLAAYRAIAADL
PRLLKPDGVAVLELGAGQEPAVAALVQSAGLRVLGPARADLGGIPRALCARWHGAANRTA
DEKRLGTFEGNG