Protein Info for GFF166 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13667: ThiC-associated
  • amino acids 18 to 86 (69 residues), 82.5 bits, E=1.5e-27
• TIGR00190: phosphomethylpyrimidine synthase
  • amino acids 133 to 589 (457 residues), 701.5 bits, E=1.8e-215
• PF01964: ThiC_Rad_SAM
  • amino acids 134 to 585 (452 residues), 617.6 bits, E=1e-189

Best Hits

Swiss-Prot: 86% identical to THIC_LEPCP: Phosphomethylpyrimidine synthase (thiC) from Leptothrix cholodnii (strain ATCC 51168 / LMG 8142 / SP-6)

KEGG orthology group: K03147, thiamine biosynthesis protein ThiC (inferred from 86% identity to lch:Lcho_2430)

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17)" (EC 4.1.99.17)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I (2/2 steps found)
• superpathway of thiamine diphosphate biosynthesis I (7/10 steps found)
• superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (3/7 steps found)

Isozymes

Compare fitness of predicted isozymes for: 4.1.99.17

Use Curated BLAST to search for 4.1.99.17

Protein Sequence (622 amino acids)

>GFF166 Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MNAPDKIPTASLALSREPFPASRKIHVSGQQPGVSVPMREIALTNGERVVVYDTSGPYSE
PTAAIDVTRGLPDVRGAWIAARGDTETYAGRARQALDDGVKHEARDAERIEALRASATGL
QRAPRRARSGLNVTQMHYARRGIVTPEMEYVAVRENGQREWMAEYLGDAAREARLRGNPM
GARIPEIITPEFVRDEVARGRAIIPANINHPEVEPMAIGRNFLVKVNANIGNSAVTSSIE
EEVDKLVWAIRWGADNVMDLSTGRNIHTTRDWIVRNSPVPIGTVPIYQALEKVGGVAEDL
TWAIYRDTLIEQAEQGVDYFTIHAGVRLPFIHLTANRRTGIVSRGGSIMAKWCITHHQES
FLYTHFDEICEIMKAYDVSFSLGDGLRPGSASDANDEAQFAELRTLGELTQTAWKHDVQT
MIEGPGHVPMHLIQANMDEQLKRCHEAPFYTLGPLTIDIAPGYDHIASAIGAAMIGWMGT
AMLCYVTPKEHLGLPDRDDVKQGLIAYKIAAHAADIAKGHPGARARDDAMSKARFEFRWH
DQFNLGLDPDTARDFHDETLPKDSSKEAHFCSMCGPKFCSMKITQDVRDYAEQGMAGKSA
EFKAAGGEFYVPVPSIPIKAGA