Protein Info for GFF1659 in Xanthobacter sp. DMC5

Annotation: Phosphoenolpyruvate-dependent phosphotransferase system

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 755 PF01590: GAF" amino acids 26 to 157 (132 residues), 63.8 bits, see alignment E=6.4e-21 PF13185: GAF_2" amino acids 34 to 160 (127 residues), 52.6 bits, see alignment E=1.5e-17 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 183 to 734 (552 residues), 403.1 bits, see alignment E=9.1e-125 PF05524: PEP-utilisers_N" amino acids 184 to 303 (120 residues), 78.8 bits, see alignment E=9.5e-26 PF00391: PEP-utilizers" amino acids 331 to 403 (73 residues), 70.3 bits, see alignment E=2.1e-23 PF02896: PEP-utilizers_C" amino acids 429 to 718 (290 residues), 300 bits, see alignment E=4.1e-93

Best Hits

KEGG orthology group: K08484, phosphotransferase system, enzyme I, PtsP [EC: 2.7.3.9] (inferred from 94% identity to xau:Xaut_2624)

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase, nitrogen regulation associated"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (755 amino acids)

>GFF1659 Phosphoenolpyruvate-dependent phosphotransferase system (Xanthobacter sp. DMC5)
MRGALGGPRVLLRRLREVMAEPVSAQDRLDKIVVLIAANMVAEVCSVYVLRVDQTLELYA
TEGLNRDAVHLTVMSVNEGLVGHVAREAESLALLDAQSHPEFSYRPETGEEVYNSFLGVP
ILRGGNTLGVLVVQNRARRTYTEEEIEALQTTAMVLAEMIASGELTALAKPGAEPAVRRP
LHLKGVALADGLGLGHVVLHEPRIRVTNVIADDVVKETARLDAAIGKLRHSIDLMLEDDG
LSKAGEHRDILEAYRMFAHDRGWMHKMREAVLSGLTAEGAVERVQSDTRARMMRASDPYL
RERLHDLDDLANRLLRELTGRSRSSEREALPENAILVARNMSPAALLDYDRTRIRGLVLE
EGGTTSHVTIVARALGIAAVGQVENAAGLADQGDPVIVDGQAGEVHLRPPGDVEEAYAEK
VRFRARRQAQYAALRTRPSVTKDGVHVDLNLNAGLLVDLPHIAETGAAGIGLFRTELQFM
IASAFPRISEQLKLYRAVLDAAGDRPVTFRTLDIGGDKVLPYMRTVEEENPALGWRAIRL
GLDRPGLLRSQMRALLRAGAGRELRVMFPMVAAVEEFDRARALVERELTHLRRHGHGLPD
RVYVGAMVEVPSLLFQLDEILSRADFLSVGSNDLVQFLYAADRANVRVADRFDPLSPPAL
RAFRLVAEAGIRHGKPVTLCGELASRPLEAVALAALGYRSLSVSPAAMGPVKAMLLETDV
SAARKVLLPLLEDRTGAADPRAVLTKFARDCGLPL