Protein Info for GFF1658 in Sphingobium sp. HT1-2

Annotation: Malate synthase G (EC 2.3.3.9)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01345: malate synthase G
  • amino acids 6 to 698 (693 residues), 1050.2 bits, E=0
• PF20656: MS_N
  • amino acids 18 to 70 (53 residues), 45.2 bits, 1.1e-15
• PF20658: MSG_insertion
  • amino acids 158 to 193 (36 residues), 44.1 bits, see alignment (E = 3.8e-15)
• PF01274: MS_TIM-barrel
  • amino acids 315 to 544 (230 residues), 223.7 bits, E=4e-70
• PF20659: MS_C
  • amino acids 567 to 649 (83 residues), 76.7 bits, E=4.2e-25

Best Hits

KEGG orthology group: K01638, malate synthase [EC: 2.3.3.9] (inferred from 80% identity to swi:Swit_3838)

Predicted SEED Role

"Malate synthase G (EC 2.3.3.9)" in subsystem Photorespiration (oxidative C2 cycle) (EC 2.3.3.9)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• glyoxylate cycle (6/6 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• glycolate and glyoxylate degradation II (1/2 steps found)
• chitin deacetylation (2/4 steps found)
• D-xylose degradation IV (3/7 steps found)
• superpathway of glycol metabolism and degradation (3/7 steps found)
• L-arabinose degradation IV (3/8 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (4/14 steps found)
• superpathway of pentose and pentitol degradation (8/42 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.3.9

Protein Sequence (704 amino acids)

>GFF1658 Malate synthase G (EC 2.3.3.9) (Sphingobium sp. HT1-2)
MTDRMTDRAGLKVAATLADFIEQRALPGTGIDADAFWAGTADILARFTPDNAALLRTRDD
LQTQIDAWHQVRRGQAHNAAAYQAFLREIGYLVEEPAPFEIGSGNVDAEVARMAGPQLVV
PILNARFLLNAANARWGSLYDALYGTDAIAGAASGKGYDAARGAQVIAWAKAFLDDAVPL
ATGSWADLAGEEIILADPAQFVGTSAKGRLFRHNGLHIEVVFDPAHPIGATDPAGISDVI
LESALTTICDLEDSVAAVDAADKVTAYANWLGLMQGDLTETFEKGGKSMTRALNPDRAYS
APDGSSFTLPGRSLLFVRNVGHLMTTPAIALPDGQDAPEGIIDGIVTSLIALHDLAKADG
NSRAGSVYIVKPKMHGPQEAAFTNRLFDAIEDMLGMARHTIKVGVMDEERRTSANLAACI
HAVRDRIVFINTGFLDRTGDEMHTSMQAGPMIRKGEMKASDWITAYEDRNVQIGLACGLS
GRAQIGKGMWAAPDRMADMLEQKIGHPQSGANTAWVPSPTAATLHATHYHRVDVFARQEE
RKAEGIASLDRLLTIPVAVGRNFSEEEIAQELDNNAQGILGYVVRWIDQGVGCSKVPDIH
DVGLMEDRATLRISSQHMANWLLHGVCTAEQVDAALTRMAAKVDAQNEADPLYQPMSGRE
GESLAFQAARALVFEGVAQPNGYTEPLLHAYRARAKAEEVLEIA