Protein Info for GFF1656 in Xanthobacter sp. DMC5

Annotation: NADH pyrophosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF09297: Zn_ribbon_NUD
  • amino acids 141 to 171 (31 residues), 40.3 bits, see alignment (E = 2.1e-14)
• PF00293: NUDIX
  • amino acids 176 to 282 (107 residues), 74.4 bits, E=8.9e-25

Best Hits

KEGG orthology group: K03426, NAD+ diphosphatase [EC: 3.6.1.22] (inferred from 78% identity to xau:Xaut_2627)

Predicted SEED Role

"NADH pyrophosphatase (EC 3.6.1.22)" in subsystem Nudix proteins (nucleoside triphosphate hydrolases) (EC 3.6.1.22)

MetaCyc Pathways

• NAD salvage pathway II (PNC IV cycle) (5/5 steps found)
• NAD salvage pathway I (PNC VI cycle) (6/7 steps found)
• NAD salvage pathway III (to nicotinamide riboside) (2/3 steps found)
• NAD salvage (plants) (6/11 steps found)

KEGG Metabolic Maps

• Nicotinate and nicotinamide metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.1.22

Protein Sequence (312 amino acids)

>GFF1656 NADH pyrophosphatase (Xanthobacter sp. DMC5)
MSFISRPDLGAWPTLGYVDSPLDRACHLRASAADLLDHPSARFYLLGGELVALKGADPVF
DPLFDRAEAEARGRGEALFLGIEGDGAPRFAMGFDPGLRETLEGEGLTVTDLRTIAVSGL
IAPQHLGPVACGKALRAWHTRHGFCANCGAASRIVDAGWRRDCPSCGAQHFPRTDPVVIM
LTARGDRCLLGRQPHFAPGMWSTLAGFVEPGETIEDAVRRETLEEAGVQTGKVRYLASQP
WPFPMSLMIGCIAEATSEKLVIDRNELEDARWFSRDEVTLMLTRTHPDGLFVPPPIAIAH
HLIRNFIEGGHA