Protein Info for GFF1645 in Sphingobium sp. HT1-2

Annotation: 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00275: EPSP_synthase
  • amino acids 18 to 76 (59 residues), 59.7 bits, E=1.1e-20
  • amino acids 83 to 455 (373 residues), 281.2 bits, E=6.7e-88
• TIGR01356: 3-phosphoshikimate 1-carboxyvinyltransferase
  • amino acids 83 to 462 (380 residues), 344.8 bits, E=3.2e-107

Best Hits

Swiss-Prot: 70% identical to AROA_NOVAD: 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) from Novosphingobium aromaticivorans (strain ATCC 700278 / DSM 12444 / CIP 105152 / NBRC 16084 / F199)

KEGG orthology group: K00800, 3-phosphoshikimate 1-carboxyvinyltransferase [EC: 2.5.1.19] (inferred from 88% identity to sjp:SJA_C1-21510)

Predicted SEED Role

"5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19)" in subsystem Chorismate Synthesis or Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) (EC 2.5.1.19)

MetaCyc Pathways

• superpathway of aromatic amino acid biosynthesis (18/18 steps found)
• superpathway of L-tryptophan biosynthesis (13/13 steps found)
• superpathway of L-phenylalanine biosynthesis (10/10 steps found)
• superpathway of L-tyrosine biosynthesis (10/10 steps found)
• chorismate biosynthesis I (7/7 steps found)
• chorismate biosynthesis from 3-dehydroquinate (5/5 steps found)
• chorismate biosynthesis II (archaea) (8/12 steps found)
• superpathway of chorismate metabolism (41/59 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Phenylalanine, tyrosine and tryptophan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.19

Protein Sequence (464 amino acids)

>GFF1645 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) (Sphingobium sp. HT1-2)
VTSHNDQPSPMTFTAAPALSGSVAVPGDKSISHRSLMLSALAVGESRVEGLLEGEDVLAT
AAALRAMGAKITKVSAADGGGGRAMGADIRRDDDGIWHIHGVGVGGLLQPQEALDMGNSG
TSTRLLMGLVASHGITATFVGDASLSKRPMARVTEPLSRVGASFTTSPGDRLPLTMTGAC
PAVPLDYTLPVASAQVKSAILLAGLNTPGITRVVEPIPTRDHSERMLKGFGADLEVEVQA
DGTRIITLKGEAELKPQNIVVPGDPSSAAFPMVAALLVPGSQVTIANVGLNATRAGLIDL
LREMGGSIEIINAREVGGEPVGDLVVAASALNGVEPDPARAPSMIDEYPVAFVAAAFAHG
RSIFRGLDELRVKESDRIATMATGLRAIGVTVEELEDGIIIEGSGGQPLPGGGPIATRLD
HRIAMSFAVAGLVSRDGVTIDDMRPVATSFPTFTTLLQTLGAIV