Protein Info for GFF1640 in Sphingobium sp. HT1-2

Annotation: ATP-dependent DNA helicase UvrD/PcrA (EC 3.6.4.12)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 758 PF00580: UvrD-helicase" amino acids 17 to 282 (266 residues), 304.4 bits, see alignment E=2.7e-94 PF13245: AAA_19" amino acids 21 to 265 (245 residues), 79.5 bits, see alignment E=8.1e-26 PF13361: UvrD_C" amino acids 287 to 446 (160 residues), 127.6 bits, see alignment E=2.5e-40 amino acids 504 to 615 (112 residues), 108.9 bits, see alignment E=1.2e-34

Best Hits

KEGG orthology group: K03657, DNA helicase II / ATP-dependent DNA helicase PcrA [EC: 3.6.4.12] (inferred from 94% identity to sch:Sphch_0654)

Predicted SEED Role

"ATP-dependent DNA helicase UvrD/PcrA" in subsystem DNA repair, bacterial UvrD and related helicases

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (758 amino acids)

>GFF1640 ATP-dependent DNA helicase UvrD/PcrA (EC 3.6.4.12) (Sphingobium sp. HT1-2)
MTVPAPSPNDPPYLKGLNEPQREAVLTTEGPVLVLAGAGTGKTAALTARLAHLVATQRAW
PSEILAVTFTNKAAREMRERVGRMIGPAVEGMPWLGTFHAIAAKMLRRHAELVGLQSNFT
ILDTDDQLRLMKQLIQAEGIDEKRWPARQLAGLIDQWKNKGLTPEEVGAGEAEGYAHGKG
QKLYAAYQARLREVNACDFGDLLLHSLTILKRHRDVLEQYQQRFKYIMVDEYQDTNSSQY
LWLRLLAQTRKNICCVGDDDQSIYSWRGAEVANILRFEKDFPGAVIVRLEQNYRSTPHIL
GAASGVIAENGNRLGKTLWTDIDVGEKVRVLGVWDGPEEARRVGDEIEAIARAGGSLDEV
AILVRAQHQTREFEDRFIQIGLPYRIVGGFRFYERAEIRDALAYLRLVNQPADDLAFERI
VNVPKRGLGDKAVEKLHRLARAEGIPLALAAARILDTDELTPQARRSLGAFIGDLARWRD
RAAQLPHAELARQILDESGYTATLQAERSTESAGRLENLSELTRAMEEYETLGAFLEHVS
LVMDNEAQQDEQKLTIMTIHAAKGLEFDNVFLAGWEEGIFPSQRALDEGGLNSLEEERRL
AYVAITRARKRCTILHAANRRIYGQWTSSIPSRFVGELPPEHVDEESSMSGGASLWRANW
SERDDPFANVARGSSRGPGWQRAQSSGQFSREPVRIVEARTSAVSLGNKGRDDVSVGQRV
FHQKFGYGTIAAIEGNKLEIDFETAGRKRVMDSFVTLP