Protein Info for GFF1634 in Xanthobacter sp. DMC5

Annotation: Esterase EstA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 630 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF00657: Lipase_GDSL" amino acids 33 to 315 (283 residues), 68.2 bits, see alignment E=1.1e-22 PF03797: Autotransporter" amino acids 359 to 607 (249 residues), 137.5 bits, see alignment E=7.2e-44

Best Hits

KEGG orthology group: K12686, outer membrane lipase/esterase (inferred from 85% identity to xau:Xaut_2645)

Predicted SEED Role

"Phospholipase/lecithinase/hemolysin"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (630 amino acids)

>GFF1634 Esterase EstA (Xanthobacter sp. DMC5)
MISRSSIALAALLSTVSFGALETAQAHEFSGFYVFGDSLSDAGTYYVVIDGQLQSVRFTV
NPGQVWDMRVGEHYGFDVSPYLAIDTVNGTSTVVGGNNYAQGGACVDSGYAIPKCLAYKY
NTLGETQQLEAYLDSTGGVARPNALYSMWAGANDIFTQAILLDLGVISEEKAIEHVVAAA
NQHVENIHTLSKAGARYIIVPNLPDISKAPVTGVLPEGPFFSAAVTAYNATLYKDLRHLG
GSNIIFVDDYALVNEVIANPHMYGFTNVTDRACQQEFSITCTTNTLVAPNAQNDYLFADV
VHPTPAGHALLAEYIESIIDAPGLIGMLAESPIYVGRGIYRTLDTRIGTGTPKGFQLYTN
IDYSTYSLDPTRDHSGSDGSATAGYVGLEYGFGNGISVGALLSYASGSYNFSALSGGYDA
DMFNGTIYANARFGGAFAQLSGTIGSIDYNSIQRQFALGASLRTDMGNTSGVYYGARLAT
GYDFTFGQATLTPLAQLTYQQATVDGYAETAGNSSAMSFGNQLRELFYVTLGGQASYAFV
VSGVTIRPNVQASWNYDFLNQDRSVTAGLLTAPVTFAMPVYQPGRSWTNLAAGLTVEAAN
GLSCNFTVGEQLGQDQVSSAYVNGGIVYKF