Protein Info for GFF163 in Sphingobium sp. HT1-2

Annotation: Malate:quinone oxidoreductase (EC 1.1.5.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF06039: Mqo
  • amino acids 56 to 541 (486 residues), 714.1 bits, E=7.7e-219
• TIGR01320: malate dehydrogenase (acceptor)
  • amino acids 56 to 539 (484 residues), 633 bits, E=1.6e-194
• PF01266: DAO
  • amino acids 57 to 329 (273 residues), 52 bits, E=8.4e-18

Best Hits

Swiss-Prot: 69% identical to MQO_STRMK: Probable malate:quinone oxidoreductase (mqo) from Stenotrophomonas maltophilia (strain K279a)

KEGG orthology group: K00116, malate dehydrogenase (quinone) [EC: 1.1.5.4] (inferred from 69% identity to sml:Smlt1234)

MetaCyc: 60% identical to malate:quinone oxidoreductase (Escherichia coli K-12 substr. MG1655)
MALATE-DEHYDROGENASE-ACCEPTOR-RXN [EC: 1.1.5.4]

Predicted SEED Role

"Malate:quinone oxidoreductase (EC 1.1.5.4)" in subsystem TCA Cycle (EC 1.1.5.4)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• partial TCA cycle (obligate autotrophs) (7/8 steps found)
• TCA cycle VI (Helicobacter) (7/9 steps found)
• TCA cycle VII (acetate-producers) (7/9 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.5.4

Protein Sequence (574 amino acids)

>GFF163 Malate:quinone oxidoreductase (EC 1.1.5.4) (Sphingobium sp. HT1-2)
MTSPSSPAAPRRRPLFKKILFALAVTLLLLAIAATAFLFRPMASPAGKAAANDKPVDVLV
IGGGIMGVTLATYLQEMEPEWRIDIYERMDKVAQESSNGWNNAGTGHSGFAELNYTPEKD
DGSIATEKAVDIAEQFEISRQFWSHEVRQGRLPKPTDFINATPHMSFVWGDDNIAYLHKR
RDALVKNPLFYGMQYSQDPAQIRRWAPLVMEGRDPKQKVAATFMPIGTDVNFGVITTQLT
HALTRNSNFNLQLGHEVRGLRQNADKTWNVTVHDLKKNSDNVVKAKFVFIGAGGASLKLL
QMSGIPESKNYAGFPVGGQFLAFEGPAATSRHNVKVYGKAEAGSPPMSVPHLDARKLDGK
SVTLFGPFALQSTKFLKNGSSLDLFASIYKNNVGGMMKVGAENLDLVRYLAQQATLTDAQ
RQAELVKYYPNAKRGDWKLITAGQRVQVIKYDPKKGTVLQFGTEIVTDKDASIAALLGAS
PGASTSPAIMLEVMCRAFPQQMATSWKPKVAAMVPSYGQKLNASPDLTNRIRRMTSQTLG
LPYVEVPAGLNGATPNAGAKLPAKRDLNSEQQAL