Protein Info for PS417_08290 in Pseudomonas simiae WCS417

Annotation: potassium-transporting ATPase subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 685 transmembrane" amino acids 45 to 65 (21 residues), see Phobius details amino acids 71 to 91 (21 residues), see Phobius details amino acids 224 to 246 (23 residues), see Phobius details amino acids 257 to 281 (25 residues), see Phobius details amino acids 589 to 608 (20 residues), see Phobius details amino acids 619 to 638 (20 residues), see Phobius details amino acids 658 to 681 (24 residues), see Phobius details TIGR01497: K+-transporting ATPase, B subunit" amino acids 22 to 680 (659 residues), 972.6 bits, see alignment E=9.1e-297 TIGR01494: HAD ATPase, P-type, family IC" amino acids 80 to 348 (269 residues), 101.6 bits, see alignment E=3.6e-33 amino acids 373 to 605 (233 residues), 149.8 bits, see alignment E=8.6e-48 PF00122: E1-E2_ATPase" amino acids 116 to 289 (174 residues), 102.9 bits, see alignment E=3e-33 PF00702: Hydrolase" amino acids 308 to 533 (226 residues), 86.8 bits, see alignment E=5.2e-28 PF12710: HAD" amino acids 446 to 529 (84 residues), 28.7 bits, see alignment E=3.6e-10

Best Hits

Swiss-Prot: 85% identical to KDPB_PSEAE: Potassium-transporting ATPase ATP-binding subunit (kdpB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01547, K+-transporting ATPase ATPase B chain [EC: 3.6.3.12] (inferred from 98% identity to pfs:PFLU1677)

Predicted SEED Role

"Potassium-transporting ATPase B chain (EC 3.6.3.12) (TC 3.A.3.7.1)" in subsystem Potassium homeostasis (EC 3.6.3.12, TC 3.A.3.7.1)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.12

Use Curated BLAST to search for 3.6.3.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U3Y9 at UniProt or InterPro

Protein Sequence (685 amino acids)

>PS417_08290 potassium-transporting ATPase subunit B (Pseudomonas simiae WCS417)
MNMPAKNAAPVQTQEPAKTAISALWRPALVQAFVKLDPRQLQRSPVMLVVELTAILTTVL
CFVPDTTVPTYVAVQIAVWLWFTVLFANFAEALAEGRGKARADSLKAGSEGLSARRKEAD
GSFKVVPATSLRKGDVVRVAAGEMIPGDGEVIEGIAAVNEAAITGESAPVIRESGGDRSA
VTGNTRLVSDWLLIRITANPGESTLDRMIALVEGAKRQKTPNEVALDILLIGLTLIFLLV
VVTLQPFAHFASGSLPLVFLVALLVTLIPTTIGGLLSAIGIAGMDRLVRLNVIAKSGRAV
EAAGDVHVLLLDKTGTITFGNRRCAAVVAAPGVSGKEVAEGALFASLADDTAEGKSIVEY
LRALHPQAEPSADELTAVPFSAETRLSGVDYQGRVFRKGAVDSLLAFIGQPRRDLQPALS
REIDKIAQSGGTPLLVCADGKLLGAIHLKDVVKPGIRERFAELRKLGIRTVMVTGDNPLT
AAAIAAEAGVDDVLAEATPEKKLARIRHEQNDGRLVAMCGDGANDAPALAQADVGMAMND
GTQAAREAANMVDLDSDPTKLLDVVQIGKELLVTRGALTTFSIANDVAKYFAILPALFAS
IYPQLGALNVMHLQSPQSAILSAIVFNALIIVVLIPLALRGVRVQAASAAALLRRNLLIY
GVGGILVPFVGIKAIDMLLTALHLV