Protein Info for GFF1621 in Sphingobium sp. HT1-2

Annotation: DNA gyrase subunit A (EC 5.99.1.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 911 TIGR01063: DNA gyrase, A subunit" amino acids 13 to 878 (866 residues), 1104.6 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 37 to 501 (465 residues), 505.5 bits, see alignment E=1.3e-155 PF03989: DNA_gyraseA_C" amino acids 534 to 580 (47 residues), 48.7 bits, see alignment 3.9e-17 amino acids 585 to 631 (47 residues), 26.6 bits, see alignment 3.2e-10 amino acids 643 to 687 (45 residues), 16.1 bits, see alignment 6e-07 amino acids 692 to 739 (48 residues), 51.1 bits, see alignment 7e-18 amino acids 783 to 828 (46 residues), 28 bits, see alignment 1.2e-10 amino acids 832 to 877 (46 residues), 31.5 bits, see alignment 9.6e-12

Best Hits

Swiss-Prot: 57% identical to GYRA_GRABC: DNA gyrase subunit A (gyrA) from Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1)

KEGG orthology group: K02469, DNA gyrase subunit A [EC: 5.99.1.3] (inferred from 87% identity to sjp:SJA_C1-21310)

Predicted SEED Role

"DNA gyrase subunit A (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (911 amino acids)

>GFF1621 DNA gyrase subunit A (EC 5.99.1.3) (Sphingobium sp. HT1-2)
LTDETVLADPSDISPISIVDEMKSSYLDYAMSVIVSRALPDVRDGLKPVHRRILFSAQES
GFVYNRPYRKSARLVGEVMGKYHPHGDSSIYDALARMTQDWSMRVPLIDGQGNFGSMDPD
PPAAMRYTEARLAKVATALLEDLDKDTVDFTPNYDASESEPQVLPARFPNLLVNGAGGIA
VGMATNIPPHNLGEVLRACLAYIENPAISTDELIQIVPGPDFPTAPLILGQSGARNAYHT
GRGSILMRARHEVEEGRGDRRSIVLTSIPYQVGKSGLVEKIAEAAKDKRIEGISDIRDES
SREGVRIVMDLKRDATPDVVLNQLWRNTPAQSSFPANMLAIRGGRPELLGLRDIIEAFVK
FREEVITRRTKFELNKARDRAHILLGLVVAVTNLDEVVKIIRGSASPAAARESLLAREWP
IGEIAPYLALVEAIETEVSGESYKLSDVQVRAILDLRLHRLTALGRDEIGKELAELAEAI
AEYLAILGDRVKLYAVMREEFEAIESEFATPRVSVIAPAADGIDDEDLIEREEMVVTVTV
QGYIKRTPLESFRAQGRGGKGRSGMATKDEDAVTELFVTSTHTPVLFFSTAGKVYRLKVW
RLPEGGPATRGRPMINLLPLAPGETIQTVLPLPEDEESWKELHVMFATANGTVRRNSMDA
FANVPSNGKLAMRFDEGSDDRLIGVALLTEEDDVLLATRQGRAIRFAATDVREFQSRTST
GVRGMTLKDGDEVISLSILKGFDATTEERDDYLRAAPWKENEATPAINSAERMAAFAEAE
QFILTVCANGYGKISSAYDYRRTGRGGQGITNIDNIARNGLVVGSFPVAHEDHLMLVTDQ
AKLIRMGLSSMRVIGRNSAGVRLFNVAKDEHVVSTARIEDGEDDNAEATAEGTVEGAPEA
APETGSEGGEA