Protein Info for PS417_08235 in Pseudomonas simiae WCS417

Annotation: CDP-glucose 4,6-dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 361 TIGR02622: CDP-glucose 4,6-dehydratase" amino acids 9 to 353 (345 residues), 509.4 bits, see alignment E=2.5e-157 PF01370: Epimerase" amino acids 15 to 247 (233 residues), 99.1 bits, see alignment E=7.1e-32 PF04321: RmlD_sub_bind" amino acids 16 to 173 (158 residues), 27.7 bits, see alignment E=3.7e-10 PF16363: GDP_Man_Dehyd" amino acids 17 to 326 (310 residues), 114.1 bits, see alignment E=2.4e-36 PF02719: Polysacc_synt_2" amino acids 63 to 233 (171 residues), 47.5 bits, see alignment E=3.5e-16 PF07993: NAD_binding_4" amino acids 83 to 174 (92 residues), 25.2 bits, see alignment E=2.2e-09

Best Hits

Swiss-Prot: 63% identical to RFBG_SALTY: CDP-glucose 4,6-dehydratase (rfbG) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K01709, CDP-glucose 4,6-dehydratase [EC: 4.2.1.45] (inferred from 82% identity to pfl:PFL_5095)

MetaCyc: 64% identical to CDP-D-glucose-4,6-dehydratase monomer (Yersinia pseudotuberculosis)
CDP-glucose 4,6-dehydratase. [EC: 4.2.1.45]

Predicted SEED Role

"Similar to CDP-glucose 4,6-dehydratase (EC 4.2.1.45)" (EC 4.2.1.45)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.45

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7TZH5 at UniProt or InterPro

Protein Sequence (361 amino acids)

>PS417_08235 CDP-glucose 4,6-dehydratase (Pseudomonas simiae WCS417)
MKSTVSPTFWQGKRVFLTGHTGFKGSWLALWLTSMGAQVKGFALAPPTHPALFVEAKVAQ
GMESEQGDIRDRAVIAQSMVSFNPDVLIHMAAQPLVRLSYREPVETYATNVMGTLHVLEA
ARQCPNLRAIVNVTTDKCYENREWEWGYREDEPMGGHDPYSNSKGCVELITSAYRKSFFN
DPQGAALASGRAGNVIGGGDWAEDRLIPDILGAFEKGQPVTIRNPKSTRPWQHVLEPLSG
YLVLAQHLWTHGPRFAQGWNFGPKDEDARPVEWIIDHMVQAWGTGARWQLDQDPQPHEAN
YLKLDISKARAHLHWAPSWNLETTLARIVTWHRAWLSGNDMQALCLEEINAYMLAMHASS
N