Protein Info for GFF161 in Sphingobium sp. HT1-2

Annotation: Na+/H+-dicarboxylate symporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 446 transmembrane" amino acids 20 to 38 (19 residues), see Phobius details amino acids 58 to 76 (19 residues), see Phobius details amino acids 88 to 110 (23 residues), see Phobius details amino acids 155 to 178 (24 residues), see Phobius details amino acids 199 to 219 (21 residues), see Phobius details amino acids 231 to 261 (31 residues), see Phobius details amino acids 318 to 347 (30 residues), see Phobius details amino acids 358 to 385 (28 residues), see Phobius details PF00375: SDF" amino acids 20 to 412 (393 residues), 369.1 bits, see alignment E=1.4e-114

Best Hits

Swiss-Prot: 73% identical to DCTA_AGRVS: C4-dicarboxylate transport protein (dctA) from Agrobacterium vitis (strain S4 / ATCC BAA-846)

KEGG orthology group: K11103, aerobic C4-dicarboxylate transport protein (inferred from 73% identity to avi:Avi_6225)

MetaCyc: 60% identical to C4 dicarboxylate/orotate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-121; TRANS-RXN-121A; TRANS-RXN-121C; TRANS-RXN-122A; TRANS-RXN0-451; TRANS-RXN0-517; TRANS-RXN0-553

Predicted SEED Role

"C4-dicarboxylate transport protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (446 amino acids)

>GFF161 Na+/H+-dicarboxylate symporter (Sphingobium sp. HT1-2)
MQISDFSDTPVARRPWYLHLYAQVLLAIIAGVALGHFAPHLGEAMKPVGDAFIKAVKMVI
APVIFLTIVTGIAGMRDIASVGRVAGKAFAYFLFFSTLALVVGMIVANVVRPGAGLNIDP
ATLDASKVSEYAQKAHDSTLTGFLTGIIPDSFLSAFVSGNILQVLFVAILFGVALALIGD
RGARVLDLLEDVSTAVFKLVAIVMKAAPVGAFGAMAFTIGKYGVGTLANLAFLVGTFYLT
SALFVVVILGLVARAAGFSIFGLIRYLKTELLLVLGTSSSESALPALIEKMERAGCPKSI
VGLVVPTGYSFNLDGTNIYMTLAALFIAQATGVDLSIGDQILLLGVAMLSSKGAAGVTGA
GFITLAATLSIVPSVPVAGMALILGVDRFMSECRSLTNFIGNAVATVVVSRWEGQLDQAQ
LQAAMRGDGREPSPAEPALANAVPAE