Protein Info for GFF1601 in Sphingobium sp. HT1-2

Annotation: Porphobilinogen synthase (EC 4.2.1.24)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 60% identical to HEM2_BRADU: Delta-aminolevulinic acid dehydratase (hemB) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K01698, porphobilinogen synthase [EC: 4.2.1.24] (inferred from 89% identity to sjp:SJA_C1-20920)

MetaCyc: 54% identical to porphobilinogen synthase subunit (Rhodobacter capsulatus)
Porphobilinogen synthase. [EC: 4.2.1.24]

Predicted SEED Role

"Porphobilinogen synthase (EC 4.2.1.24)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis (EC 4.2.1.24)

MetaCyc Pathways

adenosylcobalamin biosynthesis II (aerobic) (28/33 steps found)
superpathway of heme b biosynthesis from glycine (7/8 steps found)
tetrapyrrole biosynthesis II (from glycine) (4/4 steps found)
superpathway of heme b biosynthesis from glutamate (7/10 steps found)
tetrapyrrole biosynthesis I (from glutamate) (4/6 steps found)
adenosylcobalamin biosynthesis I (anaerobic) (25/36 steps found)
superpathway of bacteriochlorophyll a biosynthesis (6/26 steps found)

KEGG Metabolic Maps

Porphyrin and chlorophyll metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.24

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (357 amino acids)

>GFF1601 Porphobilinogen synthase (EC 4.2.1.24) (Sphingobium sp. HT1-2)
LFPPHRPRAGQSVRLDPLPVAAYTRGMTIASYPALRMRRTRATAWSRAMHAENRLSPSDF
IWPLFVTEGVGVEEPIAALPSVSRWSVDLMVARAKEARDAGIPCLALFPNTQADRRSDDG
AEALNPDNLMCRAIRAIKDAVPDIGVLTDVALDPYTAHGQDGLLDETGYVINDATIDMLI
GQSLNQAAAGADIIAPSDMMDGRIGAIREALEETGHANVQIMAYAAKYASAFYGPFRDAV
GSRGLLKGDKKNYQMDPANGEEALREVALDLAEGADSVMVKPGLPYLDIVARVRDRFSVP
VFAYQVSGEYAMIEHGAAAGSGDRDALILETLMAFKRAGCSGVLTYHALHAARLLNG