Protein Info for GFF160 in Sphingobium sp. HT1-2

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 585 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details transmembrane" amino acids 291 to 310 (20 residues), see Phobius details PF02518: HATPase_c" amino acids 480 to 583 (104 residues), 69.5 bits, see alignment E=1.6e-23

Best Hits

Swiss-Prot: 42% identical to DCTB_RHILE: C4-dicarboxylate transport sensor protein DctB (dctB) from Rhizobium leguminosarum

KEGG orthology group: K10125, two-component system, NtrC family, C4-dicarboxylate transport sensor histidine kinase DctB [EC: 2.7.13.3] (inferred from 42% identity to bsb:Bresu_3004)

Predicted SEED Role

No annotation

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (585 amino acids)

>GFF160 hypothetical protein (Sphingobium sp. HT1-2)
MDSARFPNRILWIALFLGVAGGLLWLSGEVAERNAIARLSAETRGAAILRQAYLKSEIER
FRLLPIALSDDSDLVAGLTGSQAALRALDRKLQALARTTGAAQIYVLDASGRTVASSNWG
SERSFVGQDYSFRNYFKLAAAHGSGAQFALGTISERPGMYLSRRSRGGGYVVVKLEFDGI
EQQWARLGEITFITSREGIILITSRPDWRFTTIHPLSAQAKQRFRAELQSGRGSLLPVPL
RGMGDADGRQIVSPADEPRRRLLWQSVAAGEPGWQLNMLTPLHGVESFVRTVRLATLLLL
LLLAGGIWILRERGHRRAERAAMAAARTSELEAMVTDRTRELRREMEERAASETRADTLR
EGLRQANRLASLGQITAGIAHETAQPVAAIRSYAANGRLLIARGDSEAAAENMAAIERLT
DRIGKVTAELRGFSRKATGAIDAIPLAGPIEGSVLILKSRLARVDLHLPDLPDDLRVMAD
TIRLEQVLVNLLQNALEALEGVTAPTIRLSVALDAETVLLSVIDNGPGIDPSVQGQLFTP
FVTSRPSGLGLGLVIASDIMADMGGSLRLLPSEVGAHFQLSLKRA